FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490999
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9970.2030554033714936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTAGC200.00720467452.02367441
GGTATCA5150.043.5505181
GTATCAA13050.041.512261
AACGAGA650.001871458836.8707770
CAACGCA15550.035.1994065
TCAACGC15750.034.755974
TATCAAC15800.034.695462
ATCAACG15650.034.5521553
AACGCAG16050.034.099386
ACGCAGA16550.033.0691837
GTCTTAT751.6646063E-632.205051
CGCAGAG17150.031.711548
AGAGTAC17750.031.02744511
CAGAGTA17750.031.02744510
GCAGAGT19050.028.7293979
AGTACGG16450.026.99281513
TACGGGA5650.026.80579215
GAGTACG16900.026.47774712
GTGGTAT1901.8189894E-1225.4250411
GTACGGG18000.024.28597614
Produced by FastQC (version 0.11.5)