FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404636
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT14140.34944987593787996No Hit
GTATCAACGCAGAGTACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTA8690.2147609209264623No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7220.17843197342796985No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC7190.17769056633616387No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT7060.17447780227167134No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT6620.16360383159185046No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG6520.1611324746191639No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6480.16014393183008926No Hit
CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA5740.14185589023220868No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG5580.1379017190759102No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT4830.11936654178076099No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4500.11121106377089533No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT4500.11121106377089533No Hit
GTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTA4180.10330272145829833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCG150.002453947468.283419
ACGTTAG505.912678E-661.8402770
TTACGTT200.006960568552.477346
TGGTATC3150.046.652262
GGTATCA11900.046.2832371
GTGGTAT3050.045.986341
TATAGTG306.6435285E-445.5166445
GTATCAA30500.041.3877031
TATATAG501.9654522E-434.175492
TATCAAC36800.034.0826232
AACGCAG38700.032.7261126
GTGTCGT952.61403E-432.54751270
CCACGTA552.723369E-432.32849560
ACGCAGA40050.031.7082237
CGCAGAG40300.031.511528
CAGAGTA40700.031.20182410
AGAGTAC40700.031.11794911
GCAGAGT40900.031.049259
TACCGCC553.4611908E-431.0340758
GTAAGCG703.495405E-529.98702846
Produced by FastQC (version 0.11.5)