FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918563
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT10210.11115187526604055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29950.043.634011
GGTATCA11100.041.0984651
TCGACGT2050.038.5440329
TATCAAC36800.035.4148522
TCAACGC37500.034.8452344
ATCAACG37750.034.5236173
CAACGCA38300.034.1173975
CTCGACG2350.033.61435328
AACGCAG38950.033.5480426
CGTATGC2300.033.13907241
ACGCAGA40250.032.547947
CGCAGAG40750.032.232748
AGAGTAC41250.031.59261911
CGACGTC2550.031.0015930
AACGCTA450.004757928730.485775
CAGAGTA43200.030.32533610
TCGTATG2550.029.84582940
CTCGTAT2350.029.41911339
GCAGAGT44600.029.373429
AGTACGG33700.028.5973713
Produced by FastQC (version 0.11.5)