FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479939
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGGGGTTACTACTGGACCTGGATCCGCCAGCC7520.15668657891940435No Hit
GTAATAAACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA5630.11730657437716042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG605.4005795E-946.18812640
CGTATGC551.414046E-744.1163741
GTATCAA12750.042.473821
GGTATCA5700.039.33421
TAACGGC655.3275653E-737.255136
TATCAAC14900.036.341262
TTAACGG709.539126E-734.58317635
ATCAACG15950.034.165123
TCAACGC16200.033.6378864
CAACGCA16450.033.1266675
AACGCAG16550.032.9265066
ACGCAGA17100.031.8608267
CGCAGAG17900.030.6295178
AGAGTAC18050.030.37498311
CGCGGTA802.6318667E-630.36817243
TTATACA1151.7043931E-929.9908472
TGTCGAC1301.4551915E-1029.95122166
CAGAGTA18550.029.55624410
GCCGTCT952.998404E-729.26640946
GCCATTA1055.424551E-428.73823470
Produced by FastQC (version 0.11.5)