FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562430
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12460.2215386803691126No Hit
GTAATATATGGCCGTGTCCGCAGCGGTCACGGAGCTCAACCTCAGGGAGA11200.1991358924666181No Hit
GTATGGTACCTCAGCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT10420.18526749995555003No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10270.18260050139572925No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA9600.17068790782852977No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9240.1642871112849599No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8350.1484629198300233No Hit
CTATTACACTGGGAGCCCCAACTACAATCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACAAGTCCAAGAG8190.14561812136621446No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8040.1429511228063937No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7750.1377949255907402No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7650.13601692655085967No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT7540.1340611276069911No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGTAGACTGGAGCCTGAAGATT7480.13299432818306278No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7400.13157192895115838No Hit
GTACCATACAGCTGACAGTAAAACACTGCAAAATCTTCAGGCTCCAGTCTACTGATAGTGAGAGTGAAGTCTGTC7360.13086072933520618No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7360.13086072933520618No Hit
GTGTAATAGAGATATCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCG7130.12677133154348097No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7130.12677133154348097No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6980.12410433298366019No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6950.12357093327169603No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6690.11894813576800668No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC6680.11877033586401864No Hit
GTTTTACTGTCAGCTGTATGGTACCTCAGCTTGGACGTTCGGCCAAGGGA6550.11645893711217396No Hit
GTCTCTGGTGCCTCCGTCAGTACTTACTACTGGAGCTGGATCCGGCAGCC6510.11574773749622176No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC6350.11290293903241293No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6050.10756894191277137No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCAGCTACTTAGCCTGGTACC5820.10347954412104617No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5810.10330174421705812No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT5790.10294614440908204No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT5730.10187934498515372No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5650.10045694575324929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG250.00903537649.12714469
AATTTCG306.321898E-445.98047328
GTATCAA10150.039.1417661
TATCAAC10850.036.2657472
TATACTG501.8785575E-434.4424325
ATCAACG11650.034.0171133
TCAACGC11750.033.7096184
AACGCAG11950.033.433666
CAACGCA11900.033.2847065
GGTATCA4400.032.1913951
ATTTCGG553.2630362E-431.35032529
AGAGTAC12750.031.33586311
CGCAGAG12950.030.8519128
TTAGAGT450.004658605430.6154984
TAAACTA605.4719346E-428.7020265
GCAGAGT14150.028.2354939
ACGCAGA14300.027.9393167
TACACGA500.007371366527.87111553
CCCGTAG500.007794876627.5539478
GTATTGG658.583238E-426.5745091
Produced by FastQC (version 0.11.5)