FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056515
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATATTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAACTGTGGCTGCACCAT92560.8760878927417026No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63920.6050079743307004No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55530.525595945159321No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43220.4090807986635306No Hit
CTCCTAAGTTGCTCATTACCTGGGCATCTACCCGGGAATCCGGGGTCCCT43140.40832359218752223No Hit
ACTTAGGAGCCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGAATTGTATAAAACACT42770.4048215122359834No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42570.4029284960459624No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41200.3899613351443188No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAACTGTGGCTGCACCA40130.37983369852770665No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37200.35210101134389954No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37120.35134380486789113No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33510.31717486263801276No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33340.3155657988764949No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31280.29606773211927895No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31220.29549982726227264No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28690.27155317245850746No Hit
ATATAATACTGCTGACAGTAATAAAGTGCCACATCAGCAGCCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTC28640.2710799184110022No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28140.2663473779359498No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27820.26331855203191623No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27230.25773415427135443No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26660.2523390581297947No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26000.24609210470272544No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCGC25780.2440097868937024No Hit
GAATTCACTCTCGCCATCAGCAGCCTGCAGGCTGCTGATGTGGCACTTTATTACTGTCAGCAGTATTATATTCCT24770.23445005513409653No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24250.229528213040042No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA24180.2288656573735347No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24120.2282977525165284No Hit
TTATTACTGTCAGCAGTATTATATTCCTCCGTACACTTTTGGCCAGGGGA23490.22233475151796234No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22890.21665570294789946No Hit
GTGCAGCCACAGTTCGATTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG22250.21059805113983238No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21900.2072852728072957No Hit
AACCAGGACAGGCTCCTAAGTTGCTCATTACCTGGGCATCTACCCGGGAA21740.2057708598552789No Hit
ATATTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGA21700.20539225661727473No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21290.20151157342773174No Hit
CTTTATTACTGTCAGCAGTATTATATTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAA21130.19999716047571497No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCGCCATCAGCAGCC21110.19980785885671284No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20920.19800949347619298No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20550.19450741352465417No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGTAATGAGCAACTTAGGAGCCTGTCCTGGTTTCTGCTGGTA20460.19365555623914474No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA19860.18797650766908183No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19350.18314931638452836No Hit
GTTTTATACAATTCCAACAATAAAAACTACTTAGCTTGGTACCAGCAGAA18810.17803817267147176No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18360.1737788862439246No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18060.17093936195889317No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGAATTG17930.16970890143537953No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17800.1684784409118659No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17450.16516566257932921No Hit
GGCCAAAAGTGTACGGAGGAATATAATACTGCTGACAGTAATAAAGTGCC16940.16033847129477574No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16500.15617383567672963No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16380.15503802596271704No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16240.15371291462970238No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16190.15323966058219712No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16190.15323966058219712No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16170.15305035896319505No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT15980.1512519935826751No Hit
GCTCATTACCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAATT15860.15011618386866254No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15780.14935897739265416No Hit
ATTATATTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAT15580.1474659612026332No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15570.14737131039313214No Hit
CTGCTGATGTGGCACTTTATTACTGTCAGCAGTATTATATTCCTCCGTAC15560.1472766595836311No Hit
GGTAATGAGCAACTTAGGAGCCTGTCCTGGTTTCTGCTGGTACCAAGCTA15480.1465194531076227No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15400.14576224663161433No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15350.14528899258410907No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15220.14405853206059543No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14880.1408404045375598No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14790.13998854725205037No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14570.1379062294430273No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14550.13771692782402523No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14540.13762227701452417No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14530.13752762620502312No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAATTCCAA14480.1370543721575179No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14470.13695972134801684No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14430.13658111811001264No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTGATCTCCAGCTTGGTCC14400.13629716568150949No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14320.1355399592055011No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14210.13449880030098957No Hit
GTACCAGCAGAAACCAGGACAGGCTCCTAAGTTGCTCATTACCTGGGCATCTACCCGGGAATCCGGGGTCCCTGA13440.12721068796940888No Hit
CAGTAATAAAGTGCCACATCAGCAGCCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTCCCAGACCCGCTGCCA13340.1262641798743984No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAATTCCAACAATAAAAACTA13330.12616952906489734No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCGCCATCAGCAGC13100.12399256044637322No Hit
GTAATAAAGTGCCACATCAGCAGCCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTCCCAGACCCGCTGCCA12970.1227620999228596No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12950.1225727983038575No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12940.12247814749435644No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG12940.12247814749435644No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12890.1220048934468512No Hit
GCTTGGTACCAGCAGAAACCAGGACAGGCTCCTAAGTTGCTCATTACCTGGGCATCTACCCGGGAATCCGGGGTC12710.12030117887583233No Hit
GTCTGGGACAGAATTCACTCTCGCCATCAGCAGCCTGCAGGCTGCTGATG12670.11992257563782814No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12650.11973327401882605No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC12630.11954397239982396No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCGCCATCAGCAGCCTGCAGGCTGCTGATGTGGCA12620.1194493215903229No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12580.11907071835231872No Hit
GCCTGCAGGCTGCTGATGTGGCACTTTATTACTGTCAGCAGTATTATATTCCTCCGTACACTTTTGGCCAGGGGA12530.11859746430481347No Hit
GCAGAAACCAGGACAGGCTCCTAAGTTGCTCATTACCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT12420.11755630540030194No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGTAAT12360.11698840054329565No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG12350.11689374973379459No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG12170.11519003516277573No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12140.1149060827342726No Hit
TTACCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAATTCACT11980.11339166978225582No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11170.1057249542126709No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA11120.10525170016516566No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA11100.10506239854616356No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11080.10487309692716147No Hit
GAGGAATATAATACTGCTGACAGTAATAAAGTGCCACATCAGCAGCCTGCAGGCTGCTGATGGCGAGAGTGAATT11010.10421054126065414No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAACTGTGGCTG10910.10326403316564366No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10770.10193892183262897No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10670.1009924137376185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA150.002376411868.8463125
GTATCAA21200.042.3553851
GGTATCA8350.040.3778881
GTGGTAT2550.037.776511
TATCAAC23500.037.6243672
ATCAACG24400.036.0955853
TCAACGC24600.035.6622774
CAACGCA24850.035.1650545
GTCTAGT501.8921713E-434.4036061
AACGCAG25550.034.336286
TCGTATG1056.220944E-1032.95564340
ACGCAGA27400.031.8893787
TGTTAGA14050.031.72721370
CGTATGC1001.400258E-831.16088541
CGCAGAG28050.031.1504068
TGGTATC3250.030.6986032
ACGGGTA450.00468513730.58243216
AGAGTAC28950.030.06317111
CAGAGTA29550.029.56916810
GCAGAGT30000.029.124259
Produced by FastQC (version 0.11.5)