FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056515
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATATTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAACTGTGGCTGCACCAT97510.9229400434447215No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59980.5677155553872875No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45250.4282949129922433No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44030.4167475142331155No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40880.38693250924028527No Hit
ACTTAGGAGCCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGAATTGTATAAAACACT39350.3724509353866249No Hit
CTCCTAAGTTGCTCATTACCTGGGCATCTACCCGGGAATCCGGGGTCCCT37830.35806401234246554No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAACTGTGGCTGCACCA37750.3573068058664572No Hit
ATATAATACTGCTGACAGTAATAAAGTGCCACATCAGCAGCCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTC37000.35020799515387857No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36490.34538080386932507No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36240.3430145336317989No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33420.3163230053525033No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30760.29114589002522445No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30520.2888742705971993No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28340.2682403941259708No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27590.26114158341339216No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27530.26057367855638586No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27420.25953251965187435No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26750.2531909154153041No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26400.2498781370827674No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24900.23568051565761014No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24210.22914960980203783No Hit
TTATTACTGTCAGCAGTATTATATTCCTCCGTACACTTTTGGCCAGGGGA23930.22649938713600848No Hit
GAATTCACTCTCGCCATCAGCAGCCTGCAGGCTGCTGATGTGGCACTTTATTACTGTCAGCAGTATTATATTCCT23360.22110429099444873No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCGC23270.2202524337089393No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22670.21457338513887642No Hit
CTTTATTACTGTCAGCAGTATTATATTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAA22540.2133429246153628No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21990.20813713009280513No Hit
GTGCAGCCACAGTTCGATTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21870.2070013203787925No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21860.2069066695692915No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT21840.2067173679502894No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21800.2063387647122852No Hit
ATATTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGA21140.20009181128521603No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20480.19384485785814684No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCGCCATCAGCAGCC20180.1910053335731154No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19260.18229745909901895No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19060.180404442908998No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19020.18002583967099378No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA19010.17993118886149273No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18830.17822747429047386No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGTAATGAGCAACTTAGGAGCCTGTCCTGGTTTCTGCTGGTA18680.17680771214795815No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18220.17245377491090993No Hit
GTAATAAAGTGCCACATCAGCAGCCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTCCCAGACCCGCTGCCA18130.1716019176254005No Hit
AACCAGGACAGGCTCCTAAGTTGCTCATTACCTGGGCATCTACCCGGGAA18040.17075006033989104No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17520.16582821824583655No Hit
CAGTAATAAAGTGCCACATCAGCAGCCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTCCCAGACCCGCTGCCA17420.16488171015082606No Hit
GGCCAAAAGTGTACGGAGGAATATAATACTGCTGACAGTAATAAAGTGCC17350.16421915448431873No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGAATTG17340.16412450367481768No Hit
GTTTTATACAATTCCAACAATAAAAACTACTTAGCTTGGTACCAGCAGAA17220.1629886939608051No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT16510.15626848648623068No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16500.15617383567672963No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16430.1555112800102223No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16410.1553219783912202No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA16150.15286105734419292No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16130.15267175572519084No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16030.15172524763018036No Hit
ATTATATTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAT15750.149075024964151No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15440.1461408498696185No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15420.1459515482506164No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15270.1445317861081007No Hit
GGTAATGAGCAACTTAGGAGCCTGTCCTGGTTTCTGCTGGTACCAAGCTA15060.14254411910857867No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14840.1404618012995556No Hit
CTGCTGATGTGGCACTTTATTACTGTCAGCAGTATTATATTCCTCCGTAC14550.13771692782402523No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14550.13771692782402523No Hit
CATCAGCAGCCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGA14470.13695972134801684No Hit
GAGTGAATTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG14450.13677041972901474No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14440.1366757689195137No Hit
GCTCATTACCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAATT14440.1366757689195137No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14250.13487740353899375No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14010.1326057841109686No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14000.13251113330146758No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13580.12853579930242354No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13490.12768394201691408No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13460.12739998958841095No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13430.1271160371599078No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13310.12598022744589524No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13070.12370860801787008No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12980.12285675073236063No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12830.1214369885898449No Hit
GCCTGCAGGCTGCTGATGTGGCACTTTATTACTGTCAGCAGTATTATATTCCTCCGTACACTTTTGGCCAGGGGA12700.1202065280663313No Hit
GAGGAATATAATACTGCTGACAGTAATAAAGTGCCACATCAGCAGCCTGCAGGCTGCTGATGGCGAGAGTGAATT12610.11935467078082185No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12550.11878676592381555No Hit
GTCTGGGACAGAATTCACTCTCGCCATCAGCAGCCTGCAGGCTGCTGATG12530.11859746430481347No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12500.11831351187631031No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAATTCCAA12440.11774560701930403No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12330.1167044481147925No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12330.1167044481147925No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12230.11575794001978201No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG12170.11519003516277573No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12120.11471678111527048No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11900.11263446330624742No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11530.10913238335470864No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAACTGTGGCTG11310.10705006554568558No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11220.10619820826017615No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG11210.10610355745067508No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA11050.10458914449865833No Hit
GTACCAGCAGAAACCAGGACAGGCTCCTAAGTTGCTCATTACCTGGGCATCTACCCGGGAATCCGGGGTCCCTGA10990.10402123964165202No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10970.10383193802264994No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10940.10354798559414678No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG10720.10146566778512373No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAATTCCAACAATAAAAACTA10690.10118171535662059No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCGCCATCAGCAGC10620.10051915969011326No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCGCCATCAGCAGCCTGCAGGCTGCTGATGTGGCA10620.10051915969011326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGA305.833721E-446.74014358
GTGGTAT3250.041.3099941
GTATCAA20800.039.8866541
GGTATCA8500.036.4499931
TATCAAC23700.034.817822
ATCAACG24900.033.1210253
TCAACGC25050.033.0489234
CAACGCA25500.032.3309945
TGGTATC3750.032.0904242
AACGCAG26050.031.648386
GTGCTAC553.2986765E-431.2954481
ACGCAGA26950.030.5914767
CGCTTAA450.004685015430.58259229
GTATTGG802.8086051E-630.1218681
AGAGTAC28150.029.4094311
CGCAGAG28400.029.0295878
CAGAGTA29100.028.44932710
GCAGAGT29900.027.6881459
CTTCGAT809.31584E-426.20627869
TGTTAGA14600.024.9893270
Produced by FastQC (version 0.11.5)