FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63657
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT5240.8231616318708076No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT1410.2214995994156181Illumina Paired End PCR Primer 2 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT1160.18222662079582763Illumina Paired End PCR Primer 2 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT780.12253169329374616No Hit
ATGTAGCTCTTGGCCCCCATGCTTCCCGGGATGATCCCCAGCTCGCCGGC670.10525158270103838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.10525158270103838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)