FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63657
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12631.9840708798718127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGA200.003300517363.2824669
GTCGATT200.006605553.12146445
AGTCGAT200.006605553.12146444
TTGCTCC200.006892937752.55308538
CTCATGA200.006892937752.55308538
ATACTTT200.00788547550.7971429
GATACTT200.00791033250.7567628
CACGAAC200.0080103450.59588227
CGATACT200.0080103450.59588227
GCGATAC200.00806069650.51582326
GCCTGAG200.00823879650.2376121
CGGGGTG705.7480065E-1043.0608117
ACGGGCA1050.041.4659616
GGGTGGT651.4415491E-841.22060419
ATGGTCC350.001548255938.27627620
GTATCAA6900.035.1136631
TACGGGC1750.034.44864715
CAAAGTG400.002834756233.83783728
CCGCGAT400.00294711433.5709824
TCCGCGA400.002970002833.51811223
Produced by FastQC (version 0.11.5)