FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602596
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16090.2670113973541145No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15410.25572688832982626No Hit
GTAATATATGGCCGTGTCCGCAGCGGTCACGGAGCTCAACCTCAGGGAGA12700.2107548008947952No Hit
CTATTACACTGGGAGCCCCAACTACAATCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACAAGTCCAAGAG11360.18851768017046247No Hit
GTATGGTACCTCAGCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT11040.18320732298256212No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10830.17972240107800252No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10200.1692676353643237No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9470.15715338302942602No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9410.1561576910566947No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9390.15582579373245092No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGTAGACTGGAGCCTGAAGATT9060.1503494878824287No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC8860.14703051463999098No Hit
GTCTCTGGTGCCTCCGTCAGTACTTACTACTGGAGCTGGATCCGGCAGCC8860.14703051463999098No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8810.14620077132938156No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8520.14138826012784683No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCAGCTACTTAGCCTGGTACC8490.14089041414148118No Hit
GTACCATACAGCTGACAGTAAAACACTGCAAAATCTTCAGGCTCCAGTCTACTGATAGTGAGAGTGAAGTCTGTC8430.13989472216874987No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8330.138235235547531No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8070.133920570332362No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG8000.1327589296975088No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7820.12977185377931483No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7770.1289421104687054No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT7550.1252912399020239No Hit
ATATATTACTGTGCGGGCGTTGGGATTTTTGGAGTGGTTCCCCCTCACTGGGGCCAGGGAACCCTGGTCACCGT7540.12512529123990201No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7510.12462744525353636No Hit
GTTTTACTGTCAGCTGTATGGTACCTCAGCTTGGACGTTCGGCCAAGGGA7410.12296795863231752No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC7200.1194830367277579No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7120.11815544743078281No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7120.11815544743078281No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7100.11782355010653905No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7010.11633001214744207No Hit
CATTTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA6840.11350888489137No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6770.1123472442565168No Hit
GAGTATTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG6740.11184939827015114No Hit
CACCTGCACTGTCTCTGGTGCCTCCGTCAGTACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA6730.11168344960802927No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT6600.10952611700044475No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC6570.1090282710140791No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA6450.10703688706861646No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6300.10454765713678817No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6080.10089678657010667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)