FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111810
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCTTCAGACTGGAGGCCACTGATGGCCAGGGAGG90760.8163265306122449No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC68050.612065011108013No Hit
GATTATTACTGTGCAACATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTA64180.5772569054064993No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA55320.4975670303379175No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT53590.4820068177116593No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC49460.4448601829449277No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT48880.43964346426097983No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC46680.41985591063221234No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG42250.3800109730979214No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC40600.36517030787634575No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC38800.34898049127099057No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC36160.32523542691646956No Hit
GACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGG34340.3088657234599437No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG32590.293125623982515No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTC31270.28125309180525454No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30730.27639614682364794No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGGC30010.26992022018150585No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC29990.2697403333303352No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG29930.2692006727768234No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC29790.26794146481862907No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA27360.24608521240139952No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG26560.238889738354575No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC25260.22719709302848506No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG24990.2247686205376818No Hit
GTCAGGGACCGCTGAGGGCCGCTGATGATCATCATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGGCGGTA24840.22341946915390218No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24370.2191921281513928No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT23960.2155044477023952No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23830.2143351831697862No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT23180.20848886050674123No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA22910.20606038801593796No Hit
GCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA22890.20588050116476736No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22650.20372185895071998No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC22340.20093361275757546No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTCAGCTGGTACCGCCAGCTCCCAGGAACGGCCCC22010.19796547971326034No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21490.19328842158282442No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20900.18798175947329132No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20870.18771192919653537No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC20850.18753204234536475No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20640.18564323040807332No Hit
ATCCCATGTTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGGCCAC20140.181146059128808No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC19960.17952707746827246No Hit
TCTTCAGACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT19940.17934719061710186No Hit
GGCCATCAGTGGCCTCCAGTCTGAAGATGAGGCTGATTATTACTGTGCAA19900.1789874169147606No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19900.1789874169147606No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19760.17772820895656632No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT19730.1774583786798104No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACT19480.17520979304017772No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG19030.17116233888883892No Hit
GAAGAAATACTGTCAGCTGGTACCGCCAGCTCCCAGGAACGGCCCCCAAA18740.16855397954686502No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATGATGATCATCAGCGGCCCTCAGCGGTCCCTGACCGATTCTCTGG18740.16855397954686502No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG18520.16657522418398826No Hit
GTCTGAAGATGAGGCTGATTATTACTGTGCAACATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGAC18470.16612550705606174No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCAGTCTGAAGATG18380.16531601622579398No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18260.1642366951187703No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTCAG17960.1615383923512111No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGGCGGTACCAGCTGACAGTATTTCTT17950.1614484489256258No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17870.16072890152094332No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG17850.1605490146697727No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCGCTGAGGGCCGCTG17800.16009929754184618No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17760.15973952383950496No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC17720.1593797501371637No Hit
ATGTTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGGCCACTGATG17720.1593797501371637No Hit
TCATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGGCGGTACCAGCTGACAGTATTTCTTCCGATGTTGGAG17480.15722110792311636No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG17300.15560212626258083No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA17230.15497252228348368No Hit
CATCTATGATGATCATCAGCGGCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC16950.1524541063670951No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC16910.15209433266475386No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGG16880.15182450238799794No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16830.15137478526007142No Hit
GTCATCCCATGTTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGGC16800.1511049549833155No Hit
ACATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGACCAAGGT16800.1511049549833155No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT16750.15065523785538895No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16560.14894631276926812No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG16530.14867648249251222No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16490.14831670879017098No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC16370.1472373876831473No Hit
GCCCCCAAACTCCTCATCTATGATGATCATCAGCGGCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAAGT16210.14579829287378238No Hit
GTGCAACATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGACC16200.14570834944819708No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT16180.14552846259702645No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG15990.14381953751090565No Hit
GCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG15970.14363965065973502No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG15740.14157095187127297No Hit
GACATAACCATTCAGGCTGTCATCCCATGTTGCACAGTAATAATCAGCCT15630.14058157418983458No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15480.139232422806055No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14910.1341056475476925No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA14620.1314972882057186No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14540.13077774080103613No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG14380.12933864599167125No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14360.12915875914050062No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14320.1287989854381594No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13970.12565096554267366No Hit
CTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG13730.1234923233286263No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG13660.12286271934952915No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13630.12259288907277323No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC13630.12259288907277323No Hit
GTTCCGAAGACATAACCATTCAGGCTGTCATCCCATGTTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGG13560.12196328509367607No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG13500.12142362454016424No Hit
GGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTCAGCTGGTACCGCCAG13440.12088396398665238No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13390.12043424685872586No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC13370.12025436000755525No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13080.11764600066558135No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13050.11737617038882543No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12980.11674656640972828No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12870.11575718872828991No Hit
GTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTCAGCTGGTACCGCCAGCTCCCAGGAACGGCCCCCAAA12680.11404826364216906No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG12670.11395832021658377No Hit
GGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGT12610.11341865966307194No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12540.11278905568397478No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12300.11063041346992741No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12250.11018069634200088No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC12140.1091913186605625No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12100.10883154495822128No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA12080.10865165810705066No Hit
GGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGGCTGTCATCC12030.10820194097912414No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11960.10757233700002698No Hit
GCTCCAACATCGGAAGAAATACTGTCAGCTGGTACCGCCAGCTCCCAGGA11930.10730250672327105No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA11880.10685278959534453No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG11740.10559358163715023No Hit
CAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC11700.105233807934809No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11680.1050539210836384No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11450.10298522229517633No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11450.10298522229517633No Hit
GACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACAT11430.10280533544400573No Hit
GTATTTCTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT11330.10190590118815265No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA11320.10181595776256734No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11190.10064669322995835No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC11160.10037686295320243No Hit
CAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG11150.10028691952761713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1700.060.9515721
TGGTATC1800.057.497972
TTGCTCG200.00707585552.26598754
ACTTACC200.00732597851.8088381
GGTATCA6100.043.598691
GTATCAA16000.041.878811
TATCAAC17900.037.0042842
ATCAACG19200.034.6425863
GCTTGTA501.8489898E-434.5392271
TCAACGC19350.034.008654
CAACGCA19450.033.8322755
GCCAGAG35750.033.79995370
AACGCAG19500.033.7455256
GGGCAGT1850.033.6057321
AGAGTAC20750.032.7088711
CGCAGAG20650.032.0330738
GCAGAGT21000.031.9913659
CAGAGTA21200.031.8520710
CGGATTA450.00422426431.23669664
GAGTACT4000.031.00701512
Produced by FastQC (version 0.11.5)