FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031965
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCTCACTTTCGGCGGCGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT29810.2888663859723925No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24870.24099654542547472No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA24420.23663593242018868No Hit
GTAATATACAGCCGTATCCTCGACTCTCAGGCTGTTCATTTGTAGATACA22730.22025940802255892No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19830.19215767976627113No Hit
GTCCCAGACCCACTGCCACTGAAGCTGGCTGGGATACCAGTGGCCCTAGT19710.19099484963152819No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18820.1823705261321847No Hit
GTATATTACTGTGCGAGAGAATCCATTTCAGGAAATGGGTATGAGAAGTA17830.17277717752055544No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17220.16686612433561215No Hit
GTTATTATACTGTTGACAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTGTCGATGGTGAGAGTGAACT16970.1644435615548977No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15760.1527183576962397No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14970.145063059309182No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14510.14060554379266738No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14090.1365356383210671No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13690.13265953787192394No Hit
GTCTGGGACAGAGTTCACTCTCACCATCGACAGCCTGCAGTCTGAGGATT13660.1323688303382382No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13410.12994626755752375No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13290.1287834374227808No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13220.12810511984418074No Hit
CTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTTCCAGCAGAAGCCTGACCAGGCTCCCAGGCT12840.12442282441749479No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAATCCCTGAATCTGAATCCAAAGCCAACACA12500.12112813903572311No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12410.1202560164346659No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11750.11386045069357972No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAAGCTGGCTGGGATACCA11720.11356974315989399No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11670.1130852306037511No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11650.11289142558129393No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11600.11240691302515105No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT11520.11163169293532242No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCAC11390.1103719602893509No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11350.10998435024443659No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11250.1090153251321508No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11110.10765868997495069No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10960.10620515230652203No Hit
GAGTTCACTCTCACCATCGACAGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAACAGTATAATAACTGG10910.10572063975037914No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGTCAGGCTTCTGCTGGAACCAGGC10890.10552683472792197No Hit
GTAATAAACTGCAAAATCCTCAGACTGCAGGCTGTCGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA10710.10378258952580756No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10600.1027166619022932No Hit
CATTTATGGTGCATCCACTAGGGCCACTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA10570.10242595436860746No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTT10470.10145692925632169No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10470.10145692925632169No Hit
CTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10390.10068170916649305No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10370.1004879041440359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG306.279875E-446.04638736
TCGTATG1300.042.58299340
GTATCAA20450.041.4303361
GGTATCA7900.039.6726231
CTCGTAT1400.039.5221139
ACCGATC1850.039.1218127
TATACAC2050.038.6393283
CGTATGC1750.037.5844141
GCCGTAT6300.037.16728611
ACGAGAC2050.036.96652222
TATCTCG1500.036.83711236
AGCCGTA6450.036.3029310
CGTATCC6450.036.3029313
CCGTATC6650.036.24673512
GTGGTAT3050.036.1348951
AATATAC6550.035.7538833
CGACTCT6650.035.7393121
GTATCCT6600.035.47786314
CGATCAG2050.035.31189729
GTAATAT6750.035.206431
Produced by FastQC (version 0.11.5)