FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031965
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCTCACTTTCGGCGGCGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT30430.2948743416685643No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23110.22394170344924488No Hit
GTAATATACAGCCGTATCCTCGACTCTCAGGCTGTTCATTTGTAGATACA22600.2189996753765874No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA22120.2143483548376156No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20320.1969059028164715No Hit
GTCCCAGACCCACTGCCACTGAAGCTGGCTGGGATACCAGTGGCCCTAGT19030.18440547886798486No Hit
GTATATTACTGTGCGAGAGAATCCATTTCAGGAAATGGGTATGAGAAGTA18730.1814984035311275No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18140.17578115536864136No Hit
GTTATTATACTGTTGACAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTGTCGATGGTGAGAGTGAACT17850.1729709825430126No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17190.16657541680192642No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16190.15688516567906857No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15930.15436570038712552No Hit
GTCTGGGACAGAGTTCACTCTCACCATCGACAGCCTGCAGTCTGAGGATT14380.13934581114669586No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14200.13760156594458145No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14190.13750466343335288No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13970.13537280818632413No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13470.13052768262489522No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13140.12732989975435213No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12710.12316309177152326No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12230.11851177123255148No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11830.11463567078340835No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11600.11240691302515105No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11600.11240691302515105No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11560.11201930298023674No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11480.11124408289040812No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11420.11066266782303663No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAAGCTGGCTGGGATACCA11380.11027505777812233No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT11340.10988744773320801No Hit
CTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTTCCAGCAGAAGCCTGACCAGGCTCCCAGGCT11270.10920913015460797No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAATCCCTGAATCTGAATCCAAAGCCAACACA11130.10785249499740786No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCGCCGCCGAAAGTG11110.10765868997495069No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGTCAGGCTTCTGCTGGAACCAGGC10790.10455780961563618No Hit
GAGTTCACTCTCACCATCGACAGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAACAGTATAATAACTGG10760.10426710208195046No Hit
GTAATAAACTGCAAAATCCTCAGACTGCAGGCTGTCGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA10610.10281356441352177No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10580.10252285687983603No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10410.10087551418895022No Hit
CATTTATGGTGCATCCACTAGGGCCACTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA10350.10029409912157873No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10350.10029409912157873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA200.00697709452.4518155
GGTATCA10000.042.0557171
CCGTAAA350.001342531539.4597137
GTGGTAT2800.039.3964581
GTATCAA21900.038.249641
CGTATTA400.002617176634.44345530
TATCAAC24850.033.674592
TCAACGC24750.033.351164
ATCAACG24900.033.0329323
CAACGCA25000.033.0096445
AACGCAG25650.032.4412546
CGTATCC6100.031.00291313
CCGTATC6150.030.75085412
CCGTATT450.00466524230.6089829
AATATAC6450.030.4133133
GACGTCT5850.030.10366467
AGAGTAC27850.030.00203311
GCCGTAT6350.029.78232611
CGCAGAG28100.029.6127478
AGCCGTA6550.029.39790210
Produced by FastQC (version 0.11.5)