FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602170
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7500.040.360071
GTATCAA17000.038.8492621
CGGGTAT451.018143E-438.2229317
TATCAAC19350.034.30892
CAACGCA19300.034.219565
AACGCAG19900.033.1878136
ATCAACG20400.032.3743823
TCAACGC20500.032.2164574
ACGCAGA21000.031.44947
CGCAGAG21800.030.4530858
AGAGTAC21850.029.91111811
CAGAGTA22250.029.52798510
AGTACGG19650.028.88363513
GCAGAGT23050.028.8016159
GTGGTAT3500.028.501021
GAGTACG20550.027.61865812
GGTCGCA500.00784480627.51822711
ACGGGTA1404.838512E-1027.0268316
TGGTATC3450.026.9200042
TACGGGT3600.026.7538315
Produced by FastQC (version 0.11.5)