FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602170
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6520.1082750718235714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17500.039.9642031
GGTATCA7150.036.6201971
GTGGTAT3250.034.9819261
GTACGAC601.37065945E-534.3917923
TATCAAC20850.033.6663062
CAACGCA21100.032.911175
AACGCAG21700.031.9985286
TCAACGC21750.031.930274
TGGTATC3350.031.8411122
ATCAACG22500.031.029043
ACGCAGA23100.030.0592237
AGAGTAC24300.028.71627811
CGCAGAG24350.028.657318
CAGAGTA24500.028.34155510
GTCTTAT500.0077848527.5615181
GCAGAGT25950.027.022859
CAATTCG658.3564705E-426.69697842
AGTACGG22000.025.46849613
GAGTACG22350.025.0696612
GTACGGG24400.022.963414
Produced by FastQC (version 0.11.5)