FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043728
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGATTTAGGGAGA39620.37960081553814784No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTCGTTACTACTGGGGCTGGATCCG32900.31521622491683654No Hit
GTGTATTACTGTGCGAGAGAGGACCAACTGCTTGACCTCTTCTTTGACCT30540.2926049698772094No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30520.29241334907178884No Hit
ATCCATTACAGTGGAACCACCTACCACAACCCGTCCCTCAAGCATCGAAA28480.2728680269188907No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24310.23291508898870203No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24160.23147793294804775No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24090.23080726012907576No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21320.20426777857832693No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21180.20292643294038296No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTCGTTACTACTGGG19310.18500988763355972No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCCGTGGATCTGGGACAGATTTCA18910.18117747152514832No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18850.18060260910888662No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18710.17926126347094262No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18670.1788780218601015No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18490.17715343461131636No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18430.17657857219505463No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTT18300.17533303695982094No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17780.17035089601888614No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16970.1625902533993531No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16610.15914107890178283No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA16170.1549254211825303No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16160.15482961077982002No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16150.15473380037710974No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15930.15262597151748347No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGCTGTGACATCCAGATGACCCAGTC15540.14888936581178236No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15140.145056949703371No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15110.14476951849524014No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14900.14275750003832416No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14860.14237425842748302No Hit
GTAATGGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAACGACTA14690.1407454815814082No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14680.1406496711786979No Hit
CTCCCAGATGGGTCCTGTCCCAGCTGCACCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACC14530.13921251513804364No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGATTTAGGGAGAACTGGTTTTTGGACGTGTCTA14530.13921251513804364No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14480.1387334631244922No Hit
GTCTCCATCCTCCCTGTCTGCATCTATTGGAGACAGAGTCACCATCACTTGCCGGCCAAGTCAGGGCATTAGCAC14280.13681725507028653No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGCTGTGACAT14080.13490104701608083No Hit
ATTGGAGACAGAGTCACCATCACTTGCCGGCCAAGTCAGGGCATTAGCACCTATTTAAATTGGTATCAGCAGAAA14010.13423037419710881No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13230.12675716278570662No Hit
GAGTTACCATAACCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT13210.12656554198028605No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12890.12349960909355694No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12570.12043367620682784No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12460.1193797617770147No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAG12310.11794260573636042No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12140.11631382889028558No Hit
GTATTACTGTGCGAGAGAGGACCAACTGCTTGACCTCTTCTTTGACCTCTGGGGCCAGGGAGCCCTGGTCACCGT12120.11612220808486502No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12010.11506829365505189No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12000.1149724832523416No Hit
GGATTGGGACTATCCATTACAGTGGAACCACCTACCACAACCCGTCCCTC12000.1149724832523416No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT11950.11449343123879017No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC11580.11094844633850966No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11390.10912804868701423No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11250.10778670304907026No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11170.10702021982738799No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA11140.10673278861925713No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCCGTGGA11130.10663697821654684No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG10940.10481658056505144No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT10830.10376266613523831No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTCGTTACT10720.10270875170542518No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGCTGTGACATCCAGATGACCCAGTCTCCATCCTCC10630.1018464580810326No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10590.10146321647019148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)