FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177527
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76490.649581708105207No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61260.520242847934697No Hit
GCTTAGGAGGCTGACCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAAAACACT61170.5194785342501701No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCT57840.49119892792267184No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54430.46223993165337185No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50130.4257227222815273No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48670.41332385584364517No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47060.39965113326488483No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45350.3851291732588722No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG43040.36551178868934636No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC39880.33867588598817694No Hit
GTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGA38430.32636194329302004No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37490.3183791114768494No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37470.3182092639913989No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36320.3084430335779986No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT36220.30759379615074645No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAATA36200.307423948665296No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT36160.3070842536943951No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34650.2942607685428869No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33570.28508900432856316No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33240.2822865208186309No Hit
CTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAAAACAC33010.2803332747359508No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32580.2766815537987664No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA32400.27515292642971245No Hit
GTATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGG30870.26215959379275383No Hit
GTTTATTACTGTCAGCAGTATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAA30670.26046111893824936No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30220.2566395505156145No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29790.25298782957843No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA29640.25171397343755175No Hit
ATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT28800.2445803790486333No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28500.24203266676687665No Hit
GTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT27460.2332005975234538No Hit
ATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAGCAGCC27230.23124735144077374No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25850.2195278749446934No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25800.21910325623106733No Hit
GTTTTATTACGCTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAG25230.2142626028957298No Hit
GACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAA25180.21383798418210367No Hit
GCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGACCGATT24930.2117148906139732No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24890.21137519564307228No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT24540.20840286464768962No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG24480.2078933221913383No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCACCAATAGGAACTA24310.20644961856500954No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23880.2027978976278251No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCAC23610.20050495657424416No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT23430.1989763292051902No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTT23350.19829693926338843No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC23100.19617384569525792No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC22840.1939658283844022No Hit
CTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCC22470.1908236499035691No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22440.19056887867539343No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTTTAGTACTCCCCCCTCGAT22410.19031410744721777No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22360.18988948873359168No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA22000.18683223399548374No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC21970.1865774627673081No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21930.18623776779640722No Hit
GGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAA21820.1853036066264298No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCAC21780.18496391165552892No Hit
CCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCAT21270.1806328007765427No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG20980.17817001223751133No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20770.1763866136402817No Hit
GGCCGAAGGTGATCGAGGGGGGAGTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGG20620.17511275749940342No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG20330.17264996896037202No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20300.17239519773219636No Hit
GTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGACCTGGTCTCTGCTGGTA20280.17222535024674593No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19970.16959271422226413No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG19860.1686585530522867No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19340.16424251843057527No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTTTAGTACTCCCC19340.16424251843057527No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC19210.1631385097751474No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19170.16279881480424654No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC19150.16262896731879609No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19020.16152495866336822No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18790.15957171258068817No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18470.15685415281348114No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18260.15507075421625152No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTTTAGTACT18250.1549858304735263No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18000.1528627369053958No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA17840.15150395702179228No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17840.15150395702179228No Hit
ACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATT17610.14955071093911224No Hit
GCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTT17500.1486165497691348No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17440.14810700731278348No Hit
GACCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGT17250.14649345620100432No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA17240.1464085324582791No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17200.1460688374873782No Hit
CTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCA17170.14581406625920254No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17100.14521960006012602No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17090.1451346763174008No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAG17060.14487990508922513No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16910.14360604894834683No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16670.14156787912294155No Hit
CCACTGAATCGGTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGACCTGGT16670.14156787912294155No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16500.14012417549661282No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15980.13570814087490138No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT15960.13553829338945095No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15860.13468905596219877No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15610.13256596239406823No Hit
GTCTATTACTGTGCGAGACAACGGGTCTCGGGAACTCGGGGAGTCCCTGT15270.12967855514141077No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15120.12840469900053247No Hit
GGTCTGGGGCAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT14990.1273006903451046No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC14780.12551729174787501No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14760.12534744426242456No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC14650.12441328309244713No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14620.12415851186427147No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC14620.12415851186427147No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG14620.12415851186427147No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14170.12033694344163659No Hit
CTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAAT14120.11991232472801049No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14000.11889323981530783No Hit
GTGTTTTATTACGCTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTG13770.11693999373262778No Hit
GAATCGGTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGACCTGGTCTCTG13650.11592090881992516No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13630.1157510613344747No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCACCAATAGGAACTACT13550.11507167139267295No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13540.11498674764994772No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13390.11371289150906942No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA13330.11320334905271812No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGG13330.11320334905271812No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG13260.11260888285364158No Hit
GCTAAGTAGTTCCTATTGGTGGAGCGTAATAAAACACTCTGGCTGGACTT13250.11252395911091634No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13060.11091040799913716No Hit
GAACTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGC12680.10768330577557882No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG12580.10683406834832662No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12570.1067491446056014No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12520.1063245258919753No Hit
GCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCACTGAATCGGT12510.10623960214925009No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT12440.10564513595017354No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCACCAATAG12350.10488082226564656No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT12330.10471097478019613No Hit
TAATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA12270.10420143232384481No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12250.10403158483839436No Hit
GGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGG12190.10352204238204304No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12180.10343711863931783No Hit
GTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGT12040.10224818624116475No Hit
GGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGA12010.10199341501298909No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11990.10182356752753866No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGC11950.10148387255663777No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11930.10131402507118732No Hit
GAGTGAAATCTGCCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCC11800.10021001641575948No Hit
GGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACAT11790.10012509267303424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)