FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177527
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73340.6228307291467626No Hit
GCTTAGGAGGCTGACCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAAAACACT55220.46894890732866423No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCT53050.45052045515729156No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC52610.4467838104773818No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52000.44160346217114343No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47120.4001606757212361No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45680.3879316567688044No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45650.3876768855406288No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45100.3830060796907417No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAATA39750.33757187733274907No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37760.3206720525304303No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA36680.31150028831610654No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC36630.31107566960248045No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT36560.3104812034034039No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35400.3006300492472784No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35380.30046020176182797No Hit
GTATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGG33830.2872970216394189No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33470.284239766901311No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32380.274983078944262No Hit
GTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGA32320.2744735364879107No Hit
GTTTATTACTGTCAGCAGTATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAA32180.2732846040897576No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT31480.2673399420989922No Hit
CTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAAAACAC31450.26708517087081657No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31030.26351837367635733No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30780.2613952801082268No Hit
GTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT30680.26054604268097464No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30510.2591023390546459No Hit
ATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT29900.25392199074840743No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29500.25052504103939865No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27600.2343895299216069No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27060.229803647814445No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC26980.22912425787264323No Hit
ATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAGCAGCC26960.2289544103871928No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26670.22649162184816143No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA26390.22411375705185527No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26140.22199066348372481No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG26030.22105650231374735No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25360.21536661155115763No Hit
CTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCC25190.21392290792482888No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT24970.21205458558487406No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC23750.20169388897239723No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23620.2005898803169694No Hit
GGCCGAAGGTGATCGAGGGGGGAGTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGG22980.1951547607825553No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTT22660.19243720101534825No Hit
GACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAA22500.19107842113174475No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA22460.19073872616084386No Hit
GAGTGAAATCTGCCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCC22260.18904025130633947No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTTTAGTACTCCCCCCTCGAT22190.18844578510726293No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21770.18487898791280372No Hit
GTTTTATTACGCTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAG21660.18394482674282628No Hit
GCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGACCGATT21600.18343528428647496No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT21590.18335036054374976No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21510.18267097060194798No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21460.1822463518883219No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21260.1805478770338175No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20750.17621676615483126No Hit
GGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAA20650.17536752872757908No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCAC20600.17494291001395298No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCAC20240.17188565527584504No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20180.17137611281949375No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCACCAATAGGAACTA19960.1695077904795389No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19900.16899824802318758No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA19560.1661108407705301No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTTTAGTACTCCCC19250.16347820474604827No Hit
CCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCAT19220.16322343351787263No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC18970.16110033994974213No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18970.16110033994974213No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC18650.1583827801825351No Hit
GTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGACCTGGTCTCTGCTGGTA18530.15736369526983246No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18450.15668430532803068No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18330.15566522041532807No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG18210.1546461355026254No Hit
GCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCACTGAATCGGT17630.14972055842456267No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG17590.14938086345366178No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTTTAGTACT17420.14793715982733305No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA17200.1460688374873782No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17150.1456442187737521No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17060.14487990508922513No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16870.14326635397744594No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16860.14318143023472074No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16770.14241711655019376No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16750.1422472690647433No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16650.1413980316374911No Hit
GCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTT16270.13817092941393275No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16250.13800108192848232No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16220.13774631070030666No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16220.13774631070030666No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT16170.13732169198668057No Hit
ACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATT16160.13723676824395534No Hit
GACCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGT16140.13706692075850488No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15920.13519859841855006No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15870.13477397970492397No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAG15770.13392474227767176No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15600.13248103865134303No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15220.12925393642778468No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14960.12704591911692897No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG14860.12619668168967674No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14830.1259419104615011No Hit
CTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAAT14760.12534744426242456No Hit
GAATCGGTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGACCTGGTCTCTG14610.12407358812154626No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14560.12364896940792015No Hit
GTCTATTACTGTGCGAGACAACGGGTCTCGGGAACTCGGGGAGTCCCTGT14340.1217806470679653No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14300.12144095209706444No Hit
CTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCA14300.12144095209706444No Hit
GCCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCCGATTCCCGGGT14240.12093140964071311No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14220.12076156215526268No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13920.1182138498735061No Hit
CCACTGAATCGGTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGACCTGGT13730.1166002987617269No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC13730.1166002987617269No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13540.11498674764994772No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13510.11473197642177208No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13510.11473197642177208No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA13210.11218426414001546No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13100.11125010297003805No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13010.11048578928551107No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT12980.11023101805733543No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12960.11006117057188497No Hit
GAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTG12820.10887223817373189No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC12450.10573005969289875No Hit
GTGTTTTATTACGCTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTG12440.10564513595017354No Hit
GGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACAT12380.10513559349382222No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12320.10462605103747091No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG12270.10420143232384481No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG12220.10377681361021872No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12170.1033521948965926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT3800.066.89754570
GTGGTAT2350.055.7113381
CTACGGA3900.044.3289369
GTCTTAC551.4856232E-743.8493541
TGGTATC3000.043.6034282
GTATCAA20600.042.9827081
GGTATCA7400.040.971211
AATAGAC3650.037.6781853
TATCAAC23500.037.6464042
ATCAACG23750.037.3493
TCAACGC23950.037.0229534
CAACGCA24400.036.3386085
AACGCAG24850.035.817346
TAATAGA3750.035.8007052
CCGTGTC3900.035.2464512
ATAGACA4100.035.206454
GTAATAG3950.034.889181
AACGTGT4050.034.73453562
TAACGGC400.002594704734.5044836
CGGGATT501.9028343E-434.37112417
Produced by FastQC (version 0.11.5)