FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874792
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27600.043.173181
CGTATGC1300.039.9976341
TCGTATG1150.039.16805640
ATCAACG31200.038.1916583
TCAACGC31700.037.480634
TATCAAC32100.036.9062922
CAACGCA32250.036.8414235
GGTATCA11000.035.691041
AACGCAG33200.035.6834956
ACGCAGA34700.034.139037
CGCAGAG35300.033.5587658
AGAGTAC35750.033.04190411
CAGAGTA36450.032.40735610
GCAGAGT38350.031.1591999
CTCGTAT1301.891749E-1029.3046239
AGTACGG30650.027.97795113
GTGGTAT4200.027.0589941
GAGTACG31600.027.02785912
TACGGGT4000.024.97092815
TATACAC2250.024.489763
Produced by FastQC (version 0.11.5)