FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874792
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14820.16941170015272203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29250.041.312731
GGTATCA12400.036.6483881
GTGGTAT4250.036.4524081
TCAACGC33800.035.6737444
TATCAAC34200.035.304942
ATCAACG34200.035.270623
CAACGCA34600.034.9481965
AACGCAG35950.033.635826
ACGCAGA37450.032.288597
CGCAGAG38350.031.6204178
AGAGTAC38300.031.4823111
CAGAGTA38850.031.12503810
GCAGAGT41000.029.6604449
GTATTAG3550.029.0934721
TAACGGC3000.028.80987536
AGTACGG33500.027.99471913
GAGTACG34750.026.9877112
CGGTACC2850.026.88189545
TTAACGG3350.024.74511735
GTACGGG39300.023.599614
Produced by FastQC (version 0.11.5)