FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489004
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19080.39018085741629926No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14940.30551897325993244No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14870.30408749212685376No Hit
ACTTATTACTGTGCGAGACATGTCCGATTCTCCTTCGATTTTACTTACAT13060.26707347997153397No Hit
GTAATAAGTAGCCGTGTCTGCGGCGGTCACAGCGCTCAGATTCAGGGAGA12710.2599160743061406No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12170.24887321985096236No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11920.24376078723282427No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTAGCCTAGAGCCTGAAGATT11790.24110232227139244No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11770.2406933276619414No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11020.22535602980752714No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10960.224129045979174No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10800.22085708910356563No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10380.2122682023050936No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10060.20572428855387687No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG10000.20449730472552374No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9860.2016343424593664No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9480.19386344487979648No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9330.19079598530891362No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9040.18486556347187344No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8730.1785261470253822No Hit
GTGCAGCCACAGTTCGATGGAGCTCCACCTTGGTCCCTCCGCCGAAAGAG8540.17464069823559727No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8500.17382270901669516No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGTGGCCCTCTC8470.1732092171025186No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8410.17198223327416545No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA8410.17198223327416545No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8400.17177773596943993No Hit
ACTACATCCCGTCCCTCAAGAGTCGAGTCACCATATCCCTTGACACGTCC8280.16932376831273363No Hit
GTTTATTACTGTCAACAGCGTAGCAACTGGCCGGAGCTCTCTTTCGGCGG8240.16850577909383155No Hit
GACTTCACTCTCACCATCAGTAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAGCGTAGCAACTGG8230.16830128178910603No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA8140.1664608060465763No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8040.16441583299932105No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7950.16257535725679137No Hit
CTATAGTCCCTTTGCCCCAGACGTCCATGTAAGTAAAATCGAAGGAGAAT7880.1611438761237127No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7780.15909890307645747No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7690.15725842733392772No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7590.1552134542866725No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7590.1552134542866725No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGGATCGGAGAGGGCCACTGGCGTCCCAGCCAGGTT7360.15051001627798546No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7280.14887403784018127No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7270.14866954053545572No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATGGAGCTCCACCTTGGTCC7260.14846504323073023No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7200.14723805940237708No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG7140.14601107557402393No Hit
CTCTTATAGTGGGACCACCTACTACATCCCGTCCCTCAAGAGTCGAGTCA6900.14110314026061138No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6800.13905816721335615No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT6620.1353772157282967No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA6600.13496822111884565No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC6480.13251425346213935No Hit
GGACTATAGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCA6440.13169626424323727No Hit
GCGTAGCAACTGGCCGGAGCTCTCTTTCGGCGGAGGGACCAAGGTGGAGCTCCATCGAACTGTGGCTGCACCAT6370.1302647831101586No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6360.13006028580543308No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6210.12699282623455024No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6200.1267883289298247No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6170.12617483701564813No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6160.1259703397109226No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA6060.12392536666366737No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6060.12392536666366737No Hit
ACCTACTACATCCCGTCCCTCAAGAGTCGAGTCACCATATCCCTTGACACGTCCACGAATCACTTCTCCCTGAAT6040.12351637205421634No Hit
GACCAAGGTGGAGCTCCATCGAACTGTGGCTGCACCATCTGTCTTCATCT6030.1233118747494908No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT6020.12310737744476527No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG5980.12228938822586319No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC5870.12003991787388243No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC5840.11942642595970585No Hit
ACCTTGGTCCCTCCGCCGAAAGAGAGCTCCGGCCAGTTGCTACGCTGTTG5830.11922192865498032No Hit
GTTCGATGGAGCTCCACCTTGGTCCCTCCGCCGAAAGAGAGCTCCGGCCA5820.11901743135025479No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5790.11840393943607823No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5770.11799494482662717No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT5760.11779044752190167No Hit
CCATCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT5690.116358966388823No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAGCACAAACCTGGCCAG5650.11554097716992089No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5610.1147229879510188No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCA5590.11431399334156775No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5440.1112465337706849No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA5410.11063304185650834No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA5410.11063304185650834No Hit
CCATATCCCTTGACACGTCCACGAATCACTTCTCCCTGAATCTGAGCGCT5370.10981505263760623No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC5340.10920156072342967No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5340.10920156072342967No Hit
GGTTTGTGCTGGTACCAGGCTAAGTAGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGAGGCTCTTTCCC5310.10858806880925309No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA5230.10695209037144891No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG5190.1061341011525468No Hit
CAGTAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAGCGTAGCAACTGGCCGGAGCTCTCTTTCGG5150.10531611193364471No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5150.10531611193364471No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5150.10531611193364471No Hit
CTATAAGAGATAGTCCCAATCCACTGCAGTCCCTCCCCTGGGGGCTGGCG5130.10490711732419365No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5080.10388463080056604No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA5060.10347563619111501No Hit
GTAGTAGGTGGTCCCACTATAAGAGATAGTCCCAATCCACTGCAGTCCCTCCCCTGGGGGCTGGCGGAACCAGCC5000.10224865236276187No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT4940.10102166853440872No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT4910.10040817662023215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)