FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489004
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19490.39856524691004575No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14200.29038617271024364No Hit
ACTTATTACTGTGCGAGACATGTCCGATTCTCCTTCGATTTTACTTACAT13340.27279940450384865No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13060.26707347997153397No Hit
GTAATAAGTAGCCGTGTCTGCGGCGGTCACAGCGCTCAGATTCAGGGAGA12980.2654375015337298No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTAGCCTAGAGCCTGAAGATT11340.23189994355874388No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11310.23128645164456732No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11210.2292414785973121No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10820.22126608371301668No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10730.21942560797048694No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10670.2181986241421338No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10640.21758513222795722No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10230.20920074273421077No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9420.19263646105144333No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9400.1922274664419923No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG9150.1871150338238542No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8880.18159360659626506No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8660.17709466589230352No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8640.1766856712828525No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8550.17484519554032277No Hit
GTGCAGCCACAGTTCGATGGAGCTCCACCTTGGTCCCTCCGCCGAAAGAG8260.1689147737032826No Hit
GACTTCACTCTCACCATCAGTAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAGCGTAGCAACTGG8230.16830128178910603No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA8060.16482482760877212No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7920.16196186534261478No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGTGGCCCTCTC7920.16196186534261478No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7880.1611438761237127No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7840.16032588690481062No Hit
GTTTATTACTGTCAACAGCGTAGCAACTGGCCGGAGCTCTCTTTCGGCGG7690.15725842733392772No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7570.15480445967722145No Hit
CTATAGTCCCTTTGCCCCAGACGTCCATGTAAGTAAAATCGAAGGAGAAT7550.1543954650677704No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7410.15153250280161307No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7390.15112350819216203No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7320.14969202705908338No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7220.14764705401182812No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7080.1447840917456708No Hit
ACTACATCCCGTCCCTCAAGAGTCGAGTCACCATATCCCTTGACACGTCC7070.14457959444094526No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7060.14437509713621974No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7010.14335261061259214No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6980.14273911869841555No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATGGAGCTCCACCTTGGTCC6920.1415121348700624No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA6920.1415121348700624No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGGATCGGAGAGGGCCACTGGCGTCCCAGCCAGGTT6910.1413076375653369No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6840.13987615643225823No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6830.1396716591275327No Hit
CTCTTATAGTGGGACCACCTACTACATCCCGTCCCTCAAGAGTCGAGTCA6760.13824017799445404No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6570.1343547292046691No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6540.1337412372904925No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC6450.13190076154796282No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6400.13087827502433516No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT6370.1302647831101586No Hit
GCGTAGCAACTGGCCGGAGCTCTCTTTCGGCGGAGGGACCAAGGTGGAGCTCCATCGAACTGTGGCTGCACCAT6280.12842430736762891No Hit
GACCAAGGTGGAGCTCCATCGAACTGTGGCTGCACCATCTGTCTTCATCT6230.12740182084400126No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6220.12719732353927576No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6090.12453885857784394No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6070.12412986396839289No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG6060.12392536666366737No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG6010.12290288014003975No Hit
GGACTATAGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCA5930.12126690170223557No Hit
ACCTACTACATCCCGTCCCTCAAGAGTCGAGTCACCATATCCCTTGACACGTCCACGAATCACTTCTCCCTGAAT5850.11963092326443138No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA5820.11901743135025479No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA5820.11901743135025479No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAGCACAAACCTGGCCAG5740.11738145291245061No Hit
CCATCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT5730.11717695560772509No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5710.11676796099827404No Hit
GTTCGATGGAGCTCCACCTTGGTCCCTCCGCCGAAAGAGAGCTCCGGCCA5660.11574547447464642No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT5620.11492748525574432No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5600.11451849064629327No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA5560.11370050142739119No Hit
ACCTTGGTCCCTCCGCCGAAAGAGAGCTCCGGCCAGTTGCTACGCTGTTG5540.11329150681794013No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5530.11308700951321463No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC5510.11267801490376357No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT5480.112064522989587No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC5400.11042854455178282No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTC5320.10879256611397861No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5310.10858806880925309No Hit
GGTTTGTGCTGGTACCAGGCTAAGTAGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGAGGCTCTTTCCC5300.10838357150452756No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCA5210.10654309576199786No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5180.1059296038478213No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT5140.10511161462891919No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA5030.10286214427693843No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA4970.10163516044858528No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT4960.10143066314385976No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT4950.10122616583913426No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC4920.10061267392495767No Hit
GTAGTAGGTGGTCCCACTATAAGAGATAGTCCCAATCCACTGCAGTCCCTCCCCTGGGGGCTGGCGGAACCAGCC4910.10040817662023215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACT200.007072128452.2684750
TAATACC200.007487207751.520024
AAGGGAT4200.051.43692870
GTATCAA10300.042.4192121
GGTATCA3700.041.841461
TATCAAC12000.036.6702162
TGTTAGA3650.036.55710670
TCAACGC12400.035.4546364
ATCAACG12450.035.323093
CAACGCA12550.034.7465365
ATGACGG400.00263360434.3959320
ATTCACT400.002640225234.3783262
CCTTAAG400.002656170634.33614314
GGGCTAA400.002656170634.3361436
AACGCAG13000.033.8078966
TCTCTTA1950.033.496832
ACGTGTC3850.032.0100263
CGCAGAG13850.031.7330428
AGAGTAC13900.031.61889611
GCGCTCA4050.031.53326232
Produced by FastQC (version 0.11.5)