FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317854
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21870.16595161527756488No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15160.1150355046917185No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14870.11283495743838089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.040.043631
GTATCAA29400.039.4080161
TATCAAC33100.035.0015642
TCAACGC34800.033.0935554
ATCAACG35250.032.8667143
CAACGCA35400.032.5326425
AACGCAG36850.031.1589576
ACGCAGA39650.028.9585787
GTGGTAT5400.028.0964581
CGCAGAG40700.028.042058
AGAGTAC40750.027.83841111
CCGTTCG500.0077550327.58561315
CAGAGTA42600.026.71040510
GCAGAGT43600.026.3350379
TCGTATG951.01938185E-525.52109540
TGGTATC6350.023.8921452
AGTACGG37300.023.85083213
GAGTACG38550.023.07745712
TTAACGG1352.621873E-723.0343335
CTTATAC2350.022.0097981
Produced by FastQC (version 0.11.5)