FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317854
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20360.1544935933722552No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13450.10205986399100354No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13270.10069400707513883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33950.041.735991
GGTATCA13200.038.1318241
TATCAAC37200.037.9595032
TCAACGC38100.036.4844174
ATCAACG38250.036.4409683
CAACGCA38850.035.957215
AACGCAG40650.034.365016
ACGCAGA43650.032.0031557
AGAGTAC44000.031.7485811
CGCAGAG44250.031.4914538
CAGAGTA46400.030.25471910
GCAGAGT46650.030.0188279
GTGGTAT6250.029.78671
AGTACGG38250.027.79572513
CGTTTTA500.0078172227.54049323
GAGTACG38900.027.33127412
TGGTATC6600.026.0918982
CGAACTA1359.555151E-925.5237129
TACGGGG22500.025.08197615
GTACGGG43100.024.667914
Produced by FastQC (version 0.11.5)