FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21377
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT1020.47714833699770787No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT750.35084436543949105No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC620.29003134209664594No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA620.29003134209664594No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC510.23857416849885393No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG510.23857416849885393No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC510.23857416849885393No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG460.21518454413622118No Hit
GTCGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTTTTTAAGTTGGTATCAGCAGAAA440.20582869439116808No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG430.20115076951864155No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT430.20115076951864155No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT420.196472844646115No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC420.196472844646115No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT410.19179491977358842No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC400.18711699490106187No Hit
GTCTTCATCTGTCGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG390.18243907002853532No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG370.17308322028348225No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTCTGGTCCCAGGGCCGAAAGTG370.17308322028348225No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT360.16840529541095572No Hit
ACATATTACTGTCAACAGTATGTTAATCTCCCATTCACTTTCGGCCCTGGGACCAGAGTGGATATCAAACGAA350.16372737053842917No Hit
GTAATATGTTGCAAAATCTTCCGGCTGCAGGCTGTTGATGGTGAAAGTAAAATCTGTCCCAGATGCACTTCCA330.15437152079337607No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG330.15437152079337607No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT330.15437152079337607No Hit
GTCTCCATCCTCCCTGTCTTCATCTGTCGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA320.14969359592084952No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC310.14501567104832297No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC310.14501567104832297No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG310.14501567104832297No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG300.14033774617579642No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG300.14033774617579642No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTCTGGTCC300.14033774617579642No Hit
GTATGTTAATCTCCCATTCACTTTCGGCCCTGGGACCAGAGTGGATATCAAACGAACTGTGGCTGCACCAT290.13565982130326987No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC280.13098189643074332No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC270.1263039715582168No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT270.1263039715582168No Hit
GGCTCACAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCATCCTCC270.1263039715582168No Hit
ATATTACTGTCAACAGTATGTTAATCTCCCATTCACTTTCGGCCCTGGGA260.12162604668569024No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA260.12162604668569024No Hit
GTGGAAGTGCATCTGGGACAGATTTTACTTTCACCATCAACAGCCTGCAGCCGGAAGATTTTGCAACATATTA260.12162604668569024No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG260.12162604668569024No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCACAGGTGCCAAATGTGACAT250.11694812181316369No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCACAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCATCCTCC250.11694812181316369No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG240.11227019694063714No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG240.11227019694063714No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA240.11227019694063714No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT240.11227019694063714No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC240.11227019694063714No Hit
GAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG230.10759227206811059No Hit
CAGTAATATGTTGCAAAATCTTCCGGCTGCAGGCTGTTGATGGTGAAAGTAAAATCTGTCCCAGATGCACTTCCA230.10759227206811059No Hit
GTTCAGTGGAAGTGCATCTGGGACAGATTTTACTTTCACCATCAACAGCC230.10759227206811059No Hit
AGTCAGGACATTAGCAACTTTTTAAGTTGGTATCAGCAGAAACCAGGGAA220.10291434719558404No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT220.10291434719558404No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT220.10291434719558404No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA200.00577134554.82499757
GCTTGAA200.00583034454.68478456
TGCTTGA200.0058897954.5452855
CCGTCTT200.006903667552.4062547
TGCCGTC200.00697107152.2781245
TATGCCG200.007038962652.15060843
ATGCCGT200.007038962652.15060844
GTATGCC200.007176221351.89745342
CGTATGC200.007176221351.89745341
TCGTATG200.007245592351.77179340
ATCTCGT200.007745260450.90892837
GGATCTC200.00789261550.66765235
AGGATCT200.0079670750.54787434
CAGGATC200.00804204650.42865833
GCCCACG200.0081935750.19190218
CATCTCA200.0081935750.19190228
GACATCT200.0081935750.19190226
TCCGAGC200.0081935750.19190213
CACATCT200.00827012550.0743567
TATACAC200.00827012550.0743563
Produced by FastQC (version 0.11.5)