EGAF00005755668 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005755668
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21377
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	212	0.991720072975628	No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT	66	0.30874304158675214	No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC	60	0.28067549235159284	No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC	54	0.2526079431164336	No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG	51	0.23857416849885393	No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT	50	0.23389624362632738	No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG	46	0.21518454413622118	No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA	45	0.21050661926369463	No Hit
GTCGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTTTTTAAGTTGGTATCAGCAGAAA	44	0.20582869439116808	No Hit
GTATGTTAATCTCCCATTCACTTTCGGCCCTGGGACCAGAGTGGATATCAAACGAACTGTGGCTGCACCAT	43	0.20115076951864155	No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC	41	0.19179491977358842	No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG	39	0.18243907002853532	No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT	37	0.17308322028348225	No Hit
GTCTTCATCTGTCGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG	37	0.17308322028348225	No Hit
GTCTCCATCCTCCCTGTCTTCATCTGTCGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA	36	0.16840529541095572	No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT	36	0.16840529541095572	No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC	35	0.16372737053842917	No Hit
GTAATATGTTGCAAAATCTTCCGGCTGCAGGCTGTTGATGGTGAAAGTAAAATCTGTCCCAGATGCACTTCCA	34	0.15904944566590262	No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT	34	0.15904944566590262	No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG	34	0.15904944566590262	No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG	33	0.15437152079337607	No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG	33	0.15437152079337607	No Hit
CAGTAATATGTTGCAAAATCTTCCGGCTGCAGGCTGTTGATGGTGAAAGTAAAATCTGTCCCAGATGCACTTCCA	33	0.15437152079337607	No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC	33	0.15437152079337607	No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT	33	0.15437152079337607	No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA	31	0.14501567104832297	No Hit
AGTCAGGACATTAGCAACTTTTTAAGTTGGTATCAGCAGAAACCAGGGAA	30	0.14033774617579642	No Hit
ATATTACTGTCAACAGTATGTTAATCTCCCATTCACTTTCGGCCCTGGGA	30	0.14033774617579642	No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCACAGGTGCCAAATGTGACAT	30	0.14033774617579642	No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG	30	0.14033774617579642	No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTCTGGTCCCAGGGCCGAAAGTG	29	0.13565982130326987	No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG	28	0.13098189643074332	No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC	26	0.12162604668569024	No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCACAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCATCCTCC	25	0.11694812181316369	No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC	25	0.11694812181316369	No Hit
ACATATTACTGTCAACAGTATGTTAATCTCCCATTCACTTTCGGCCCTGGGACCAGAGTGGATATCAAACGAA	24	0.11227019694063714	No Hit
CCCATTCACTTTCGGCCCTGGGACCAGAGTGGATATCAAACGAACTGTGG	24	0.11227019694063714	No Hit
GATGAAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGTGAGCCAGAGCAGCAGGAGC	24	0.11227019694063714	No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT	24	0.11227019694063714	No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTCTGGTCC	24	0.11227019694063714	No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT	22	0.10291434719558404	No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT	22	0.10291434719558404	No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC	22	0.10291434719558404	No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA	22	0.10291434719558404	No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATGAATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG	22	0.10291434719558404	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACAGAA	20	0.00577455	54.817307	58
GTATCAA	35	0.0015509353	38.146534	1
GAAGATG	50	1.7452618E-4	34.677616	44
ATCAGAT	40	0.0028184901	33.773697	32
TCAACGC	40	0.0029868165	33.37822	4
ATCAACG	40	0.0029868165	33.37822	3
GTGCAGC	45	0.0031542534	32.9919	61
CACAGTT	50	0.0033952398	32.48433	68
AGATGAA	45	0.0043023424	30.975279	46
GCGGGAA	45	0.004513221	30.675278	40
CAGCCAC	50	0.0048895273	30.164022	64
GATGGCG	45	0.004959486	30.092373	36
TCAGATG	45	0.005017636	30.021065	33
GGGAAGA	70	3.6660967E-5	29.57973	42
GGTGCAG	50	0.005559032	29.386597	60
TGGTGCA	50	0.005629774	29.311052	59
CCACAGT	55	0.0068291407	28.166998	67
GCCACAG	55	0.0072013307	27.864124	66
GGCGGGA	50	0.00782569	27.408655	39
TGGCGGG	50	0.008106283	27.21241	38

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content