FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469169
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTT250.0021466770.62018670
TCGTATG600.063.4883140
CGTATGC750.050.83969541
CTCGTAT750.050.76887539
CGTACTA750.050.58457629
ACTAGAT800.047.45343832
GGTATCA5050.038.176471
GTACTAG1009.094947E-1237.9546430
ATCTCCG1009.094947E-1237.86969410
GTATCAA11650.037.825371
TATACAC1100.037.5567253
CTAGATC1051.6370905E-1136.1704633
ACCGTAC1051.6370905E-1136.10485527
GACCGTA1051.6370905E-1136.10126
TCTCGTA1151.8189894E-1236.09694338
ATGCCGT1102.5465852E-1134.74545744
ATCAACG13100.033.9013943
TATCAAC13250.033.5176052
CAACGCA13200.033.3837555
TCAACGC13350.033.2665334
Produced by FastQC (version 0.11.5)