FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469169
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8010.1707273924747799No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAA300.00392079860.66224370
ATGCGAT200.007452442351.5803722
GTATCAA12850.041.281211
GGTATCA6150.039.7665631
CAACGCA14950.035.862885
ATCAACG15000.035.766213
TCAACGC15000.035.7433364
AACGCAG15350.035.376146
TATCAAC15100.035.3317382
ACGCAGA16350.033.002267
CGCAGAG16750.032.214148
AGAGTAC16700.032.10479411
CAGAGTA17300.030.99133110
GGGTCGA450.00468248930.58245725
GCAGAGT18050.029.894019
GTGGTAT2300.028.455131
GTATTAT1353.110472E-1028.0668541
AGTACGG15850.026.88773213
GAGTACG16150.026.60107612
TCGGTGT658.675023E-426.52501333
Produced by FastQC (version 0.11.5)