FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149197
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35950.041.4255871
GGTATCA14100.041.223731
TATCAAC40900.036.4103932
TCAACGC42950.034.5924534
GTGGTAT4800.034.393761
ATCAACG43300.034.392263
CAACGCA43600.034.0767365
CGTATGC1350.033.41068641
TCGTATG1253.6379788E-1233.28012540
CTCGTAT1154.5474735E-1133.1333939
AACGCAG45750.032.6256646
ACGCAGA49250.030.1661177
AGAGTAC49700.029.82378411
CGCAGAG50300.029.6047768
CAGAGTA51400.028.77048710
GCAGAGT52600.028.310279
ACTCGAC1353.1468517E-1028.04529427
TGGTATC5650.028.0007822
CTCGACG1555.4569682E-1126.6529428
AGTACGG39500.025.85949713
Produced by FastQC (version 0.11.5)