FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199725
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG43110.3593323469961866No Hit
GTAATAGACGGCCGTGTCTTCGGTTTTCAGGCTGTCCATTTGTAGATACA30070.2506407718435475No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27970.2331367605076163No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27490.22913584363083206No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24300.20254641688720335No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22450.1871262164245973No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21320.1777073912771677No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21180.1765404571881056No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20310.16928879534893412No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20210.16845527099960406No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19900.1658713455166809No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGCGGAGCCCCAGCCCCAGAATTCCCAGGTGTTTTCATTTGGTGAT18330.15278501323219904No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17870.1489508012252808No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG17640.14703369522182166No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17590.14661693304715664No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17030.14194919669090833No Hit
GTCCATGTAGTGGTCACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTC16920.14103231990664528No Hit
GTCTATTACTGTGCTAGAGCGGGGAACTACTACAGTTACTACTACTTTGA16830.14028214799224822No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16790.1399487382525162No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16330.13611452624559794No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16320.13603117381066496No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16260.13553105920106692No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15990.13328054345787577No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15790.13161349475921566No Hit
CTGTAGTAGTTCCCCGCTCTAGCACAGTAATAGACGGCCGTGTCTTCGGT15780.13153014232428265No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15740.1311967325845506No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAA15670.1306132655400196No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT15320.1276959303173644No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15280.12736252057763237No Hit
CTCTAGCACAGTAATAGACGGCCGTGTCTTCGGTTTTCAGGCTGTCCATTTGTAGATACAGTGAGTTCTTTGAAT15220.12686240596803433No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15110.12594552918377128No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14970.1247785950947092No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14920.12436183292004419No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14830.12361166100564713No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14740.12286148909125007No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACCACTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGG14710.12261143178645106No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14270.11894392464939882No Hit
GATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA14180.11819375273500177No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14080.11736022838567171No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG13890.11577653212194461No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT13760.11469295046781555No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA13360.11135885307049533No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13250.11044197628623227No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13190.10994186167663422No Hit
GTGCTAGAGCGGGGAACTACTACAGTTACTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCT12690.10577423992998394No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA12640.10535747775531892No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12410.1034403717518598No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12370.10310696201212778No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGCCTTGGTCCAGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGC12280.10235679009773073No Hit
CGGTTACACCACAGAATACGCCGCGTCTGTGAAAGGCAGATTCACCATCTCAAGAGATGATTCAAAGAACTCA12270.10227343766279773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25750.044.5746771
GTGGTAT3100.044.475361
TATCAAC30300.037.881122
ATCAACG31400.036.8833923
TCAACGC31750.036.4768034
CAACGCA32400.035.8513955
AACGCAG32900.035.4113126
AGTCGAC400.002608603634.46697210
CGCAGAG34300.033.964548
AGAGTAC34250.033.91348611
CAGAGTA35050.033.336110
CGTATGC1154.5474735E-1133.15912241
TCGTATG1154.5474735E-1133.14661440
TCTTGAG11650.032.96219370
GCAGAGT36100.032.4619679
TCACGGT14650.032.35574770
ACGCAGA37450.031.1077067
GAGTACT9550.027.79128312
ATATATC500.007769707627.5747263
AGTACTT9800.027.08232113
Produced by FastQC (version 0.11.5)