FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199725
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG39930.3328262726874909No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30150.2513075913230115No Hit
GTAATAGACGGCCGTGTCTTCGGTTTTCAGGCTGTCCATTTGTAGATACA29950.24964054262435142No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26580.22155077205192855No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23840.19871220488028507No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21000.1750401133593115No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20190.16828856612973805No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19830.16528787847214987No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19310.16095355185563356No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18450.1537852424513951No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG17870.1489508012252808No Hit
GTCCATGTAGTGGTCACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTC17430.14528329408822854No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17200.14336618808476942No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16720.13936527120798517No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16620.1385317468586551No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16600.1383650419887891No Hit
GTCTATTACTGTGCTAGAGCGGGGAACTACTACAGTTACTACTACTTTGA16480.13736481276959303No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16350.13628123111546395No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16100.13419742024213882No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16080.13403071537227282No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACCACTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGG16060.13386401050240682No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16010.1334472483277418No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGCGGAGCCCCAGCCCCAGAATTCCCAGGTGTTTTCATTTGGTGAT16000.13336389589280875No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15650.13044656067015356No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15240.12702911083790036No Hit
CTGTAGTAGTTCCCCGCTCTAGCACAGTAATAGACGGCCGTGTCTTCGGT15200.12669570109816833No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15140.1261955864885703No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14970.1247785950947092No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14940.12452853778991019No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAA14810.12344495613578113No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14800.1233616037008481No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14700.12252807935151805No Hit
GATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA14590.121611202567255No Hit
CTCTAGCACAGTAATAGACGGCCGTGTCTTCGGTTTTCAGGCTGTCCATTTGTAGATACAGTGAGTTCTTTGAAT14540.12119444039258996No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13920.11602658942674363No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13820.11519306507741357No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT13710.11427618829315052No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA13610.11344266394382047No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13360.11135885307049533No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA13300.1108587384608973No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGCCTTGGTCCAGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGC13170.10977515680676822No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12920.10769134593344308No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12910.10760799349851008No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12850.10710787888891206No Hit
GTGCTAGAGCGGGGAACTACTACAGTTACTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCT12850.10710787888891206No Hit
GGGCTGGAGTGGGTTGGCCGTACTAGAAACAAAGCTCACGGTTACACCAC12650.10544083019025194No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12570.10477401071078789No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12420.10352372418679281No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12280.10235679009773073No Hit
CGGTTACACCACAGAATACGCCGCGTCTGTGAAAGGCAGATTCACCATCTCAAGAGATGATTCAAAGAACTCA12240.1020233803579987No Hit
GTAATAAGTTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG12210.1017733230531997No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG12020.10018962678947259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2550.051.4348031
GTATCAA24650.044.5270421
TATCAAC29200.037.540132
TCTTGAG12800.036.54393870
ATCAACG31600.034.664393
TCAACGC31800.034.5417374
CAACGCA32350.033.950235
AACGCAG32950.033.4365046
TCACGGT15550.032.06020770
CTATAAC553.286955E-431.3148883
CGCAGAG35400.031.1223958
CCGCGTA450.00444630630.91159446
AGAGTAC35700.030.66798211
CAGAGTA36250.030.3926310
GCAGAGT36650.030.060929
ACGCAGA37800.029.4196177
CTCTTGA13250.026.28050669
GAGTACG32050.026.21127112
AAAATCG750.001301318924.747468
TACGGGG15800.024.40648815
Produced by FastQC (version 0.11.5)