FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237649
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14680.6177177265631246No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCA10800.45445173343881107No Hit
GTGGTATCCCAGCCAGGTTCACCGGCAGTGGGTCTGGGACAGAGTTCACT9750.41026892602114884No Hit
CTATAATGACTGGTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT8560.360195077614465No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8250.3471506297102029No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8120.3416803773632542No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8060.3391556455108164No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7860.3307398726693569No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT7490.31517069291265687No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6670.2806660242626731No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAATTTA6430.27056709685292174No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6400.2693047309267028No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC6240.26257211265353525No Hit
GTGTTAGCACCAAGTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATACATCCA6080.2558394943803677No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5570.23437927363464608No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5390.22680507807733255No Hit
GTCCCAGACCCACTGCCGGTGAACCTGGCTGGGATACCACTGGCCCTGGT5380.22638428943525957No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAAGTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT5360.22554271215111363No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5360.22554271215111363No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC5250.2209140370883109No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT5240.2204932484462379No Hit
GTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAAGTTGGCCTGGTA5200.21881009387794606No Hit
GTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG5180.2179685165938001No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5130.21586457338343526No Hit
GTCATTATAGTGCTGACAGTAATAAATTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT5040.2120774756047785No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5000.2103943210364866No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4980.20955274375234062No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4930.2074488005419758No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4930.2074488005419758No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4800.20197854819502709No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4760.20029539362673524No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4620.1944043526377136No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4470.18809252300661902No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC4440.18683015708040007No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC4430.18640936843832712No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4370.18388463658588927No Hit
GTCAGAGTGTTAGCACCAAGTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATA4260.17925596152308657No Hit
GGCCAGTGGTATCCCAGCCAGGTTCACCGGCAGTGGGTCTGGGACAGAGT4230.17799359559686764No Hit
GCACTATAATGACTGGTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT4140.17420649781821088No Hit
GGATACCACTGGCCCTGGTGGATGTATCATAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG4130.17378570917613792No Hit
GTCCACCACCAGTCATTATAGTGCTGACAGTAATAAATTGCAAAATCTTC4090.17210255460784601No Hit
CTGCTACTCTGGCTCCCAGATGCCACTGCAGAAGTAGTGATGACGCAGTC4000.16831545682918925No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCGGTGAACCTGGCTGGGATACCA3970.16705309090297035No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3950.1662115136188244No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT3760.15821652941943792No Hit
TTATAGTGCTGACAGTAATAAATTGCAAAATCTTCAGACTGCAGGCTGCT3720.15653337485114602No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3710.15611258620907303No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3690.1552710089249271No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3460.14559287015724873No Hit
ATAATGACTGGTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA3460.14559287015724873No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG3330.14012261781030005No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3300.13886025188408116No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT3290.13843946324200818No Hit
CTCCCAGGCTCCTCATTTATGATACATCCACCAGGGCCAGTGGTATCCCA3210.1350731541054244No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA3200.1346523654633514No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATACATCCACCAGGGCCAGTGGTATCCCAGCCAGGTT3180.13381078817920547No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG3110.13086526768469464No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC3090.13002369040054873No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGC3080.12960290175847575No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA3060.12876132447432978No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC3040.12791974719018384No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCACTGCAGAAGTAGTGATGACGCAGTCTCCAGCCACC2950.12413264941152709No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC2890.12160791755908924No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA2870.1207663402749433No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT2860.12034555163287033No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA2840.11950397434872437No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA2830.11908318570665141No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC2810.11824160842250546No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT2760.11613766521214058No Hit
GTATCAACGCAGAGTACGGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTT2740.11529608792799466No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC2730.11487529928592168No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCACTGCAGAAGTAGTGATGACG2720.1144545106438487No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC2710.11403372200177572No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC2710.11403372200177572No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCACTGCAGAAGTAGTGATGACGCAGTCTC2690.11319214471762978No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC2680.11277135607555681No Hit
CACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAATTTATTACTGTCAGCACTATAATGACTGGTGGTGGACGTT2660.11192977879141085No Hit
GTATCAACGCAGAGTACGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCT2650.11150899014933789No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC2650.11150899014933789No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC2590.10898425829690005No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT2590.10898425829690005No Hit
CCACTGGCCCTGGTGGATGTATCATAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCCAA2570.10814268101275411No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA2570.10814268101275411No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG2570.10814268101275411No Hit
TTATTACTGTCAGCACTATAATGACTGGTGGTGGACGTTCGGCCAAGGGA2560.10772189237068114No Hit
AAGTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCAT2560.10772189237068114No Hit
TCATTATAGTGCTGACAGTAATAAATTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC2550.10730110372860815No Hit
CATTTATGATACATCCACCAGGGCCAGTGGTATCCCAGCCAGGTTCACCGGCAGTGGGTCTGGGACAGAGTTCA2530.1064595264444622No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT2510.10561794916031626No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC2500.1051971605182433No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC2500.1051971605182433No Hit
GAGTGTTAGCACCAAGTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATACATC2410.10141006273958653No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC2400.10098927409751354No Hit
CCCTGTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAAGTTGGC2390.10056848545544059No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA2380.10014769681336762No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA2380.10014769681336762No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC2380.10014769681336762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)