EGAF00005755677 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005755677
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23879
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	56

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT	602	2.5210435947904015	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT	95	0.39783910549017965	No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT	90	0.3769002052012228	Illumina Paired End PCR Primer 2 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	89	0.3727124251434315	Illumina Paired End PCR Primer 2 (95% over 22bp)
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTG	24	0.10050672138699275	TruSeq Adapter, Index 5 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCAGCA	15	0.0026278778	66.918236	2
TACTCCC	15	0.0026278778	66.918236	22
CTTATAC	70	0.0	57.35849	1
AGGGGGG	100	5.4569682E-11	54.0	70
CGTCAGG	20	0.008207674	50.18868	14
TATACAC	80	0.0	50.18868	3
CAACCGA	20	0.008207674	50.18868	17
ACTCCCT	20	0.008207674	50.18868	23
TTATACA	80	0.0	50.18868	2
GCGTCAG	20	0.008207674	50.18868	13
TACGGGT	55	3.1723175E-9	48.66781	15
ACGGGTG	45	2.2047043E-6	44.612164	16
AAGGGGG	90	6.5847416E-10	42.11082	69
TACACAT	100	0.0	40.150944	5
AAAGGGG	90	1.544322E-9	38.64407	68
GTATCAA	200	0.0	38.47799	1
CGGGTGC	35	0.0015362249	38.238995	17
GGGTGCC	35	0.0015362249	38.238995	18
GGTGCCT	35	0.0015362249	38.238995	19
GTGCCTG	35	0.0015362249	38.238995	20