FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23879
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12535.247288412412581No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCT150.002577891367.243189
CAAGCCA150.002577891367.2431814
CCGTGCT200.00805201150.432399
AAGCCAT200.00805201150.4323915
GTGGTAT350.001454101438.667871
ACGGGCA558.468396E-636.67810416
GTATCAA1950.034.701941
GGTATCA905.7443685E-933.834391
TGGTATC400.002859414833.6922262
TACGGGC808.293864E-833.62159315
TATCAAC2300.029.2975882
AACGCAG2300.029.2361686
CAACGCA2350.028.614125
CAGAGTA2400.028.01799210
ACGCAGA2450.027.4461987
TCAACGC2450.027.4461984
TACGGGA500.00860386326.89727615
AGTACGG2000.026.89727613
CGCAGAG2500.026.8972768
ATCAACG2500.026.8972763
Produced by FastQC (version 0.11.5)