FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10992
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT5364.87627365356623No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT1281.1644832605531297TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT980.8915574963609898RNA PCR Primer, Index 3 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGTCGTATGCCGT280.2547307132459971No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT250.2274381368267831No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC220.20014556040756915No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC210.19104803493449782No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC200.1819505094614265No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG200.1819505094614265No Hit
GTGCTGCACCGCGTGGCGCAGCTGATCCGCGAAGAGGCCGAATCGCTCGC180.16375545851528384No Hit
GTGCTGGACGATGCCGATCTCGACCACGCGGTGAACGGCGTGCTCTACGG170.1546579330422125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1455604075691412No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAA150.13646288209606985No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.13646288209606985No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGC140.12736535662299855No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATACC140.12736535662299855No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.12736535662299855No Hit
ATATAGATCCTGCGCAACGCCTTGCCGAATTCAAGAGATGTTTGCCGTCCTTTGTCCGTAAGCTCTCCGGGCATA140.12736535662299855No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.11826783114992723No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG130.11826783114992723TruSeq Adapter, Index 3 (95% over 24bp)
GCCCCGGAGCCGTCTTCCGGCCGGGCCATAATCGCCAGTTCACTCATCTG130.11826783114992723No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGTAGAGG120.10917030567685589No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.10917030567685589No Hit
GTGCAGCACGGCCGCGCGCTCGTGGGGCAGGCGCTGCGCCCAGCCACTGG120.10917030567685589No Hit
GGCGTACGCCGCCCGTGCGAATCCTGCCGCCTTCGGAAACGCCGAGCTCC120.10917030567685589No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.10007278020378457No Hit
CGCCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC110.10007278020378457No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.10007278020378457No Hit
ACCTGATGGCGCTCGACAGCCGCGGCGGCCTGGTTCCGCTGCTGCAGGGCGCCTGAATCCGGCCCTGCCCCGGAG110.10007278020378457No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.10007278020378457No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.10007278020378457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1101.8189894E-1256.8140570
CTTATAC900.045.3417051
TATACAC950.042.95533
AAGGGGG1053.274181E-1141.96886469
TTATACA1000.040.8075332
AAAAAAG1155.456968E-1238.23028665
AAAGGGG1101.2369128E-1037.19967768
AAAAAGG1101.6007107E-1036.33456866
TACGGGT350.002052282435.87475615
TACACAT1150.035.484815
ATACACA1150.035.484814
AAAAGGG1152.419256E-1034.95804667
TGAAAAA1401.8189894E-1233.4797159
GAAAAAA1451.8189894E-1232.6967960
CGTATGC1403.6379788E-1232.02406741
TTGAAAA1355.0931703E-1131.8263958
CTTGAAA1355.0931703E-1131.8263957
TCTGCTT1355.820766E-1131.4687953
CTCGTAT1158.221832E-1031.29751839
TCGTATG1405.456968E-1231.1768740
Produced by FastQC (version 0.11.5)