FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10992
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG118010.735080058224163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT230.20924308588064047No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG220.20014556040756915No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC190.17285298398835516No Hit
GGTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG150.13646288209606985No Hit
GGCCTGCACCGCGCGGCCCAGCTTCCAGGCGCGCTTGAAGTCGCGGCTCC140.12736535662299855No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.11826783114992723No Hit
GTAGACGGTGAGCGCGGATGTCTTGGGGTTGGCCGCCAGCGGCTTGTTGC120.10917030567685589No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCG110.10007278020378457No Hit
GCTTGTAGGTGTTGACCCAGACCGTGCCGGCCTGCACCGCGCGGCCCAGCTTCCAGGCGCGCTTGAAGTCGCGG110.10007278020378457No Hit
GTTCTGGACATGAGGGACGATGAATTCTTGGTCGACCAGTCCTACTAGGG110.10007278020378457No Hit
GCCCCGGAGCCGTCTTCCGGCCGGGCCATAATCGCCAGTTCACTCATCTG110.10007278020378457No Hit
GCGTACGCCCGCATGGCGACGGCTGTGACCGCGGCTACTTCTACAAGCCG110.10007278020378457No Hit
ATATAGATCCTGCGCAACGCCTTGCCGAATTCAAGAGATGTTTGCCGTCCTTTGTCCGTAAGCTCTCCGGGCATA110.10007278020378457No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAG200.006145241753.7589339
AGCACGC200.007786800650.6574533
TACGGGG407.1996066E-540.0941815
AGAGAAA451.221177E-436.64319230
CAGAGAA451.2910567E-436.30232629
AGAAATG400.002887548433.4532
GTATCAA1850.031.208441
TACGGGT851.9601612E-730.18855915
GTACGGG1700.030.18855914
GGGTGCC752.6118487E-629.93698718
GGTGCCT752.6118487E-629.93698719
GTGCCTG752.6118487E-629.93698720
GAGAAAT450.005386944429.45283131
TCAACGC2050.028.1637154
TATCAAC2050.028.1637152
CGGGTGC804.319063E-628.06592617
ACGGGTG804.319063E-628.06592616
AGTACGG1850.027.74083713
AACGCAG2000.027.264046
CAGAGTA2000.027.2640410
Produced by FastQC (version 0.11.5)