FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670475
Sequences flagged as poor quality0
Sequence length21-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA28320.42238711361348297No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT27680.412841642119393No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC22390.33394235430105523No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG21470.32022073902830084No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATT21360.3185801111152541No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG21150.31544800328125583No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC20760.30963123158954475No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC19800.2953130243484097No Hit
GATTATTACTGCTGCTCATGTGCAGGTAGTGGCACGAATGTCTTCGGAAATGGGACCAGGGTCACCGTCCTCCGT19440.2899436966329841No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG18060.2693612737238525No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16150.24087400723367763No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCCAAAGC16010.23878593534434542No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCA15600.232670867668444No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15250.22745068794511356No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15130.22566091203997168No Hit
GTAATAGACGGCCGTGTCTTCAGATCTCAGGCTCCTCAACTCCATGTAGG14820.22103732428502182No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCA14820.22103732428502182No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14730.21969499235616538No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13240.1974719415339871No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13020.19419068570789366No Hit
GCCTTGGGCTGACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAG12790.19076028188970506No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12620.1882247660240874No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT12170.18151310637980536No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12160.1813639583877102No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12090.18031992244304412No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12050.1797233304746635No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11930.17793355456952162No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC11360.1694321190200977No Hit
CGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAGCAGTAATAATCAGCCTCGT11310.16868637905962192No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG11050.16480853126514783No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10790.16093068347067377No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10770.16063238748648345No Hit
GGGTCACCGTCCTCCGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG10710.15973749953391253No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC10560.15750027965248517No Hit
ACCTGCACATGAGCAGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCC10500.15660539169991425No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10430.15556135575524813No Hit
GTACCAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTCA10220.15242924792124987No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10060.15004288004772734No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG9870.1472090681979194No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9830.14661247622953877No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9780.145866736269063No Hit
GGTACCAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTC9650.14392781237182595No Hit
GTCTGGGGCTGAGGTGAAGAAGCCGGGGTCCTCGGTGAAAGTCTCCTGCAAGGCCTCTGGAGGCACCTTCAGCAA9640.1437786643797308No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG9510.14183974048249376No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC9420.14049740855363735No Hit
GACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAGCAGTAATAAT9390.1400499645773519No Hit
GTCAATAAGCGGCCGTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAAT9370.13975166859316157No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9230.13766359670382938No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCCAAAGCCCCCAAA9080.13542637682240202No Hit
CTTATTGACCTCATAAATCATGACTTTGGGGGCTTTGGCTGGGTGCTGTTGGTACCAGGAGACAAGGTTATAACC8970.1337857489093553No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8950.13348745292516498No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8890.13259256497259406No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8850.13199597300421342No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8640.12886386517021514No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8510.12692494127297813No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8500.12677579328088295No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8330.12424027741526529No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT8280.12349453745478951No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8090.12066072560498155No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8090.12066072560498155No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT8060.12021328162869609No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7980.1190200976919348No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC7970.11887094969983966No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG7960.1187218017077445No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT7920.11812520973936387No Hit
CAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTCAATAAGCGGCCGTCAGGGGTTTCTAATCG7900.11782691375517357No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7830.11678287781050749No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC7610.11350162198441402No Hit
TGTGCAGGTAGTGGCACGAATGTCTTCGGAAATGGGACCAGGGTCACCGT7480.111562698087177No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCTGATTATTACTGCTGCTCATGT7480.111562698087177No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGG7410.11051866214251091No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG7370.10992207017413028No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG7360.10977292218203513No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG7280.10857973824527387No Hit
GTCTATTACTGTGCGAGAACCTATTATTATGGTTCGGGGAGTCCACCAGT7270.10843059025317872No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC7170.10693911033222715No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA7140.10649166635594168No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA7080.10559677840337074No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7020.1047018904507998No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC6980.10410529848241919No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6980.10410529848241919No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT6910.10306126253775308No Hit
GCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTGTCAGGG6890.10276296655356276No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6870.10246467056937245No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG6870.10246467056937245No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6870.10246467056937245No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC6860.1023155225772773No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6820.10171893060889668No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG6820.10171893060889668No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG6820.10171893060889668No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC6740.10052574667213543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGC150.002308293269.3502845
TATACTG252.5733432E-455.1698655
ACCTTCG200.0070822352.2517953
GACCGTA408.291463E-751.72175226
CGGTCAT200.007377954751.71403514
GTGGTAT1250.049.6491741
GGTATCA4050.047.6741071
TCGTATG451.8198007E-646.14698840
ACCGTAC451.8662831E-645.97831727
TATACAC451.8672417E-645.9748883
CTCGTAT503.7648188E-641.5198539
ACTAGAT503.8292146E-641.4175632
GTATCAA9950.040.196151
AGGGGGG808.1316444E-539.70463670
CGTATGC557.218614E-637.7651141
CTAGATC557.3607625E-637.6579533
TTATACA557.417526E-637.6158182
ATACACA557.417526E-637.6158184
TGGTATC1500.036.779912
ATGCCGT601.2993049E-534.6621444
Produced by FastQC (version 0.11.5)