FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670475
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA27100.4041910585778739No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT26160.3901711473209292No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC23950.3572094410678996No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATT21880.32633580670420226No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG20930.3121667474551624No Hit
GATTATTACTGCTGCTCATGTGCAGGTAGTGGCACGAATGTCTTCGGAAATGGGACCAGGGTCACCGTCCTCCGT19920.29710280025355157No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19570.2918826205302211No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19250.2871098847831761No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC17440.26011409821395276No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG16520.24639248294119842No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCCAAAGC16360.24400611506767592No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15530.23162683172377793No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCA14740.21984414034826055No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCA14660.21865095641149931No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14460.21566799656959618No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14180.21149185279093183No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14000.20880718893321898No Hit
GTAATAGACGGCCGTGTCTTCAGATCTCAGGCTCCTCAACTCCATGTAGG13330.1988142734628435No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13310.1985159774786532No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12690.1892688019687535No Hit
GGGTCACCGTCCTCCGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG12150.18121481039561504No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT11990.17882844252209257No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT11960.17838099854580708No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11840.1765912226406652No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC11750.1752488907118088No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11670.17405570677504753No Hit
GCCTTGGGCTGACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAG11640.17360826279876207No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11500.17152019090942988No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11270.16808978709124128No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC11050.16480853126514783No Hit
CGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAGCAGTAATAATCAGCCTCGT10980.16376449532048176No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10810.16122897945486409No Hit
GTACCAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTCA10520.15690368768410456No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10510.15675453969200942No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC10180.15183265595286924No Hit
GTCTGGGGCTGAGGTGAAGAAGCCGGGGTCCTCGGTGAAAGTCTCCTGCAAGGCCTCTGGAGGCACCTTCAGCAA10130.15108691599239346No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCCAAAGCCCCCAAA10090.15049032402401283No Hit
GGTACCAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTC9780.145866736269063No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9510.14183974048249376No Hit
GTCAATAAGCGGCCGTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAAT9510.14183974048249376No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9470.14124314851411313No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9210.13736530071963907No Hit
ACCTGCACATGAGCAGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCC9190.13706700473544875No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9180.13691785674335358No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9110.1358738207986875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9070.13527722883030685No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG8810.1313993810358328No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8740.13035534509116672No Hit
GACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAGCAGTAATAAT8720.13005704910697638No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8720.13005704910697638No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC8560.12767068123345388No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8480.12647749729669264No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8460.12617920131250232No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG8440.125880905328312No Hit
CTTATTGACCTCATAAATCATGACTTTGGGGGCTTTGGCTGGGTGCTGTTGGTACCAGGAGACAAGGTTATAACC8330.12424027741526529No Hit
GTCTATTACTGTGCGAGAACCTATTATTATGGTTCGGGGAGTCCACCAGT8310.12394198143107497No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8090.12066072560498155No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG8010.1194675416682203No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGG7910.11797606174726873No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC7900.11782691375517357No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG7850.11708117379469779No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT7840.11693202580260263No Hit
CAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTCAATAAGCGGCCGTCAGGGGTTTCTAATCG7740.11544054588165106No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT7720.11514224989746076No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCTGATTATTACTGCTGCTCATGT7710.1149931019053656No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7690.1146948059211753No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7510.11201014206346246No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT7400.11036951415041577No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA7300.10887803422946418No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7240.10798314627689323No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT7140.10649166635594168No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC7130.10634251836384653No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6980.10410529848241919No Hit
GTGCAGGTAGTGGCACGAATGTCTTCGGAAATGGGACCAGGGTCACCGTCCTCCGTCAGCCCAAGGCCAACCCCA6910.10306126253775308No Hit
GTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCC6880.10261381856146762No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC6850.10216637458518214No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAAC6830.10186807860099183No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC6790.10127148663261121No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG6760.10082404265632575No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG6740.10052574667213543No Hit
GTCATGATTTATGAGGTCAATAAGCGGCCGTCAGGGGTTTCTAATCGCTT6720.10022745068794513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAG150.002202814770.1729761
CGATATC200.006449179353.50357467
GATTCGA200.00704172352.32762555
ATACCGT200.007360342451.74529632
TCCTATA200.007377747451.7143972
ATAGGTC200.007390821451.6912463
ACGGGAC651.1485099E-842.4133263
GTATCAA10750.040.4425931
ATTACCA350.001285829139.80553452
GTAGTTT650.001290761539.76954370
GGTATCA4100.039.5540471
TGTTAGC350.00135198239.4014432
GTGGTAT1500.039.1052061
TGGTATC1650.035.5209962
TATCAAC12400.035.0323332
CGCAGTT400.00246440834.8666554
CGCGGTA4400.033.78151770
ATCAACG13200.033.1554953
AACGCAG13250.033.0156026
TCAACGC13200.032.882164
Produced by FastQC (version 0.11.5)