FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678063
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25920.382265364722747No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGGCTGCTGTCATAGGA24470.3608809210943526No Hit
GGTTATGGTGTCCACTGGTACCAGCAGGTTCCAGGAACCGTCCCCAAACTCCTCATCTATGGTAACAACAATCGG24330.35881621619230075No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGTCCACTGGTACCAGCAGGTTCCAGGAACCGT22870.3372842936423312No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGG22660.33418723628925334No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19860.2928931382482159No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAG19810.2921557436403402No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTG19500.28758389707151105No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18290.2697389475609198No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18020.26575701667839124No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCCGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16220.2392108107948671No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15320.2259377078531051No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15240.22475787648050402No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14810.21841628285277329No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14750.2175314093233225No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14330.21133729461716685No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGTGGGTCAC13790.20337343285210963No Hit
CCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGACGGTGACCCACTGCCCTGGGGCCCCAGACA13140.1937873029497259No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC13060.19260747157712482No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12710.18744570932199514No Hit
GGCCATCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTC12620.18611839902781896No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12480.18405369412576708No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT12090.17830201618433686No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12050.17771210049803632No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11760.17343521177235743No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11640.17166546471345584No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11560.17048563334085476No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11430.168568407360378No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGTGGGTCAC11190.1650289132425748No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11160.16458647647784938No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCCGTGTTCGG11080.16340664510524833No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10860.1601621088305954No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10640.15691757255594246No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10640.15691757255594246No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC10560.15573774118334138No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9980.14718396373198359No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9930.14644656912410794No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9900.14600413235938253No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGGC9880.14570917451623228No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT9790.14438186422205607No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT9740.1436444696141804No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9650.1423171593200042No Hit
GTGTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAG9520.14039993333952744No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT9450.1393675808885015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9390.13848270735905072No Hit
TCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCCAGTCCTAT9380.1383352284374756No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9220.13597556569227343No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9070.13376338186864642No Hit
GTGCAGGAGACGGTGACCCACTGCCCTGGGGCCCCAGACACTGAGGGCGG8860.13066632451556862No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT8700.12830666177036648No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG8680.1280117039272162No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA8670.12786422500564107No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8480.12506212549571352No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8320.12270246275051137No Hit
GTTCAACTCCATGTAGGCTGTGTTCGTGGATTTGTCCGCGGTAATCGTGA8270.1219650681426357No Hit
GGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGTCCACTGGTACCAG8230.12137515245633518No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAG8130.11990036324058384No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8000.1179831372601071No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8000.1179831372601071No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAAGATG7940.1170982637306563No Hit
GTGGGTCACCGTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTT7870.11606591127963037No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7830.11547599559332983No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC7820.11532851667175469No Hit
GAGTGGTTCCGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT7790.11488607990702929No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT7690.11341129069127795No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG7620.11237893824025201No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7580.11178902255395148No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAAC7540.11119910686765094No Hit
GAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATC7340.10824952843614827No Hit
AGGTTATGGTGTCCACTGGTACCAGCAGGTTCCAGGAACCGTCCCCAAAC7330.10810204951457313No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTC7320.107954570592998No Hit
GTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGT7300.10765961274984773No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7120.10500499216149531No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7100.10471003431834504No Hit
GCTTGGTCCCTCCGCCGAACACGGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCTT7100.10471003431834504No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC7090.10456255539676991No Hit
CTCACTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCA7040.10382516078889426No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7040.10382516078889426No Hit
GCTCCAACATCGGGGCAGGTTATGGTGTCCACTGGTACCAGCAGGTTCCA7020.10353020294574398No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7020.10353020294574398No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG7000.10323524510259371No Hit
GTTCCTGGAACCTGCTGGTACCAGTGGACACCATAACCTGCCCCGATGTT7000.10323524510259371No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA6990.10308776618101857No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC6980.10294028725944344No Hit
CCCCAGGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAGCTCCAACATC6980.10294028725944344No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6930.10220289265156776No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG6900.10176045588684236No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6880.10146549804369212No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6860.10117054020054184No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC6790.1001381877495159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGA501.8166455E-434.6406544
GTATCAA16150.033.7346041
TCAACGC15550.033.623754
ATCAACG15700.033.31973
AACGCAG15900.033.316286
CAACGCA16000.032.678085
AGAGTAC16500.032.3132511
GTGGTAT1400.032.0188981
CGCAGAG16700.031.926278
TGCGTAG4350.031.53078870
CAGAGTA16950.031.45537810
TTTGCTA450.00440975730.96183652
GGGACTA850.00456780530.73589370
ACGCAGA17950.029.702997
TATCAAC18350.029.6616252
GAGTACT2200.028.14379912
GAGTACG16800.028.05073512
AGTACGG16900.027.88475213
GATTATC755.7706595E-527.86565252
TACGGGG8250.027.10343715
Produced by FastQC (version 0.11.5)