FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665096
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22910.34446155141513407No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT20720.31153397404284494No Hit
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTCCTGCATAGGAGCAGCAGTAATAAT20670.3107822028699616No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC20570.309278660524195No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC20270.30476803348689513No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16460.24748307011318668No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16390.24643059047115No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15710.23620650251993694No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT14900.2240278095192273No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG14170.21305195039513095No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTC12870.19350589990016479No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG12420.18673995934421497No Hit
GATTATTACTGCTGCTCCTATGCAGGAATTAACACTTATGTCTTCGGAACTGGGACCCAGGTCACCGTCCTAGGT12260.18433429159098838No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC12250.1841839373564117No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG12040.1810264984303018No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAATATTGTCTCCTGGTACCAACAACACCCAGGCAAAGC11840.17801941373876853No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTCCTGCATAGGAGCAG11590.174260557874352No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11220.16869745119501545No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC10830.16283363604652562No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG10820.16268328181194897No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG10540.1584733632438024No Hit
GGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10400.15636840395972912No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10360.15576698702142247No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAATATTGTCTCCTGGTACCAACAACA9800.14734714988512937No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT9600.14434006519359613No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9210.13847625004510627No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTC8890.13366491453865306No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8580.12900393326677653No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG8240.12389188929117No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC8140.12238834694540336No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT7980.11998267919217677No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGC7970.1198323249576001No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATC7630.11472028098199356No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC7500.11276567593249695No Hit
GTGTTAATTCCTGCATAGGAGCAGCAGTAATAATCAGCCTCGTCCTCAGC7390.11111177935215366No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG6980.1049472557345105No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA6900.1037444218578972No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG6810.10239123374670724No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCC6670.10028627446263397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5400.041.541881
GTATCAA12600.041.0853841
TATCAAC15000.033.5484242
ATCAACG15600.032.2338333
TCAACGC15800.032.034164
CAACGCA16000.031.844145
GTGGTAT2400.031.6357441
AACGCAG16500.030.8791646
TTACGGA450.00467003730.6009719
ACGCAGA17600.028.7536137
AGAGTAC17750.028.31667511
CAGAGTA18050.027.84603710
CGCAGAG18300.027.653758
TGGTATC2900.027.336342
GCAGAGT18550.027.2810579
TACTCCG1209.4299746E-426.14667570
AGTACGG16100.024.80389413
GAGTACG16450.024.4854312
ACGGGTG1301.8835635E-723.8334516
TATAAGA1202.4753954E-622.9783732
Produced by FastQC (version 0.11.5)