FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856035
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26220.3062958874345091No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22650.26459198514079446No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22150.25875110246660477No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21890.2557138434760261No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20450.2388921013743597No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19810.23141577155139686No Hit
CACCATCACCAGTCTGGAACCTGAAGATTTTGCTACTTATTACTGTCAACAGAGTCACAGTCCTCCCCTCACTTT16420.19181458702039053No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16330.19076322813903637No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15900.1857400690392332No Hit
GTCCTGATCTCTGTTGCATCGAATTTGCAAAGTGGGGTCCCATCAAGATT15840.18503916311833044No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15470.18071690993943004No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15240.1780301039093028No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14640.1710210447002751No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14540.16985286816543715No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14170.16553061498653676No Hit
CCCCTAAGGTCCTGATCTCTGTTGCATCGAATTTGCAAAGTGGGGTCCCA14050.16412880314473122No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13930.1627269913029257No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13560.1584047381240253No Hit
GTCTGGAACCTGAAGATTTTGCTACTTATTACTGTCAACAGAGTCACAGTCCTCCCCTCACTTTCGGCGGAGGGA13090.15291430841028697No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12620.1474238786965486No Hit
GTCCTCCCCTCACTTTCGGCGGAGGGACCACGGTGGAGGTCAAACGAACT12540.14648933746867826No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12390.14473707266642136No Hit
ACTTATTACTGTCAACAGAGTCACAGTCCTCCCCTCACTTTCGGCGGAGGGACCACGGTGGAGGTCAAACGAA12330.14403616674551858No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12240.14298480786416443No Hit
GTCACAGTCCTCCCCTCACTTTCGGCGGAGGGACCACGGTGGAGGTCAAA12160.14205026663629408No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12080.14111572540842374No Hit
GAGTCACAGTCCTCCCCTCACTTTCGGCGGAGGGACCACGGTGGAGGTCAAACGAACTGTGGCTGCACCAT12030.14053163714100475No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCGTGGTCCCTCCGCCGAAAGTG11290.13188713078320394No Hit
CTGTTGCATCGAATTTGCAAAGTGGGGTCCCATCAAGATTCAGAGGCAGTGGATCTGGGACAGATTTCACTCTCA11220.1310694072088174No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11100.12966759536701186No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGACTTGAGTGGCTGGGATGGATCAATCCTAACAGTGGTGC10930.12768169525778736No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10650.12441080096024111No Hit
GATTTCACTCTCACCATCACCAGTCTGGAACCTGAAGATTTTGCTACTTATTACTGTCAACAGAGTCACAGTCCT10630.12417716565327352No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10450.12207444789056524No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10400.12149035962314625No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10390.12137354196966245No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10310.12043900074179209No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10100.11798583001863241No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10030.11716810644424584No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9990.11670083583031068No Hit
GTGGGGTCCCATCAAGATTCAGAGGCAGTGGATCTGGGACAGATTTCACT9970.1164672005233431No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9730.11366357683973202No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9470.11062631784915337No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9360.10934132366083162No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9340.10910768835386403No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9200.10747224120509091No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9070.10595361170980158No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8870.10361725864012569No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTAAATATTTAAATTGGTATCGGCAGAAA8820.10303317037270673No Hit
CCTTAGGGGCTTCCCCTGGTTTCTGCCGATACCAATTTAAATATTTACTA8640.10093045260999843No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8630.10081363495651463No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8600.10046318199606323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)