FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671044
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19180.2858232843151865No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17720.2640661417135091No Hit
GTAATATATGGCCGTGTCCGCAGCGGTCACGGAGCTCAACCTCAGGGAGA16880.25154833364131113No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16620.24767377399991652No Hit
GTATGGTACCTCAGCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT14770.22010479193614726No Hit
CTATTACACTGGGAGCCCCAACTACAATCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACAAGTCCAAGAG13970.2081830699626254No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12510.186425927360948No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11820.1761434421587854No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11630.173312033190074No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11510.17152377489404572No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11460.17077866727070057No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGTAGACTGGAGCCTGAAGATT11370.16943747354867936No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11040.1645197632346016No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC10870.1619863973152282No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10840.16153933274122115No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10690.1593040098711858No Hit
GTACCATACAGCTGACAGTAAAACACTGCAAAATCTTCAGGCTCCAGTCTACTGATAGTGAGAGTGAAGTCTGTC10570.15751575157515751No Hit
GTCTCTGGTGCCTCCGTCAGTACTTACTACTGGAGCTGGATCCGGCAGCC10550.15721770852581948No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCAGCTACTTAGCCTGGTACC10530.15691966547648142No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9800.14604109417564273No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9780.1457430511263047No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9600.14306066368226228No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC9590.14291164215759325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9540.14216653453424813No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9470.14112338386156495No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9380.13978219013954374No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT9290.13844099641752255No Hit
GTTTTACTGTCAGCTGTATGGTACCTCAGCTTGGACGTTCGGCCAAGGGA9150.13635469507215622No Hit
GAGTATTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG9140.1362056735474872No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9080.13531154439947304No Hit
CACCTGCACTGTCTCTGGTGCCTCCGTCAGTACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA9030.13456643677612795No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8930.1330762215294377No Hit
GTGTAATAGAGATATCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCG8870.13218209238142356No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG8660.12905264036337408No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8590.12800948969069093No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8450.12592318834532462No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8400.12517808072197947No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8360.1245819946233034No Hit
ATATATTACTGTGCGGGCGTTGGGATTTTTGGAGTGGTTCCCCCTCACTGGGGCCAGGGAACCCTGGTCACCGT8300.12368786547528926No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC8170.12175058565459194No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8110.12085645650657781No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7930.11817406906253539No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7790.11608776771716907No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7650.11400146637180274No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA7590.11310733722378859No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7420.11057397130441521No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7400.11027592825507716No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7390.11012690673040813No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT7370.1098288636810701No Hit
CAGTAATATATGGCCGTGTCCGCAGCGGTCACGGAGCTCAACCTCAGGGAGAACTGGCTCTTGGACTTGTCTA7340.10938179910706303No Hit
CATTTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA7290.10863669148371792No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7220.10759354081103474No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7160.1066994116630206No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG7110.1059543040396755No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT7110.1059543040396755No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7080.10550723946566842No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT6930.10327191659563308No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA6880.10252680897228794No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA6830.10178170134894284No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT6800.10133463677493577No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6790.10118561525026674No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6720.10014246457758359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT300.003023756264.7780170
ATGCTTA200.00639698653.6135664
GACGCAA400.00943954148.58350870
GTATCAA13800.039.9963381
GGTATCA6350.038.8715821
TATCAAC16800.033.4263882
ATCAACG17050.033.3230133
TCAACGC17150.032.919334
CAACGCA17800.031.7148535
AACGCAG17900.031.5376766
TTCAGTA1053.5854016E-430.84666870
TGTTAGA4450.030.56939570
AGAGTAC18850.030.1297511
CGCAGAG19400.029.2755558
GCAGAGT19450.029.2002949
TCGTTGA953.117966E-729.13932241
AGGGTTA756.5195294E-527.3708785
ACGCAGA21200.026.7898927
ACGGGGC1800.026.61454616
ACCCTCG959.806101E-625.6457348
Produced by FastQC (version 0.11.5)