FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735018
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30220.4111463936937599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19050.042.013591
GGTATCA7050.039.1469231
ATCAACG22900.034.427783
TCAACGC23100.033.964484
TATCAAC23950.033.23082
CAACGCA23600.033.094585
AACGCAG23700.032.954946
ACGCAGA24700.031.6207337
AGAGTAC25900.030.2885211
CGCAGAG25900.030.0228338
GTGGTAT2900.029.7398511
CAGAGTA26900.029.0346510
GCAGAGT27700.028.3203169
AGTACGG23050.028.21200213
GAGTACG23550.027.7591212
TGGTATC2950.026.862112
TTGCGCG809.7347904E-525.80502518
GTACGGG25700.024.90134814
TACGGGG10700.024.75996215
GTATTAC2053.6379788E-1223.5597651
Produced by FastQC (version 0.11.5)