FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909901
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22140.2433231747190079No Hit
GTAATATATGGCCGTGTCCGCAGCGGTCACGGAGCTCAACCTCAGGGAGA20890.22958541643541439No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19840.21804569947719588No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18080.19870293581389625No Hit
CTATTACACTGGGAGCCCCAACTACAATCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACAAGTCCAAGAG17160.18859194571717144No Hit
GTATGGTACCTCAGCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT16150.17749183702402788No Hit
GTCTCTGGTGCCTCCGTCAGTACTTACTACTGGAGCTGGATCCGGCAGCC16100.17694232669268414No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15160.16661153246342186No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15010.16496300146939064No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC14240.15650054236669703No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13930.15309357831236586No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13910.15287377417982834No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13900.1527638721135596No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13210.14518062954101602No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGTAGACTGGAGCCTGAAGATT13190.14496082540847852No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12560.1380369952335474No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12410.13638846423951617No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCAGCTACTTAGCCTGGTACC12380.13605875804070994No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG12350.13572905184190368No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12260.13473993324548494No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11970.13155277332369125No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11950.13133296919115375No Hit
CACCTGCACTGTCTCTGGTGCCTCCGTCAGTACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA11680.12836561340189756No Hit
GTACCATACAGCTGACAGTAAAACACTGCAAAATCTTCAGGCTCCAGTCTACTGATAGTGAGAGTGAAGTCTGTC11370.12495864934756638No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11050.12144178322696644No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10870.119463546034129No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10830.119023937769054No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC10780.11847442743771026No Hit
ATATATTACTGTGCGGGCGTTGGGATTTTTGGAGTGGTTCCCCCTCACTGGGGCCAGGGAACCCTGGTCACCGT10630.11682589644367904No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC10360.11385854065442286No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10290.11308922619054161No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9970.10957236006994167No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT9880.10858324147352294No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9840.10814363320844796No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9800.10770402494337296No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9730.10693471047949173No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9720.10682480841322298No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9580.10528617948546051No Hit
GTTTTACTGTCAGCTGTATGGTACCTCAGCTTGGACGTTCGGCCAAGGGA9410.1034178443588918No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA9390.1031980402263543No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9310.1023188236962043No Hit
GTGTAATAGAGATATCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCG9270.10187921543112932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7700.047.9406431
GTATCAA18900.039.245281
TATCAAC21450.034.5778582
TCAACGC22550.032.585174
CAACGCA22650.032.4413035
ATCAACG22900.032.237793
AACGCAG23100.031.809336
AGAGTAC24900.029.78531511
CGTATGC703.729544E-529.70964641
CGCAGAG25050.029.606968
ACGCAGA25600.028.9708717
GTCTATA605.4219295E-428.749451
GCAGAGT26000.028.3924889
TGGATTG8600.027.95064570
TATAAGC906.9080215E-626.8313454
TAAGCAT809.5645E-525.8730836
GCTTGTA1359.424184E-925.5550671
TACGGGT1359.429641E-925.5536614
CAGTTCG8250.025.4009970
TGTTAGA2701.1332304E-925.11045670
Produced by FastQC (version 0.11.5)