FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705771
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.042.073791
GTATCAA17100.039.7855641
CGAATTA350.001375575239.26330615
CTCGTAT1400.037.0720139
TCGTATG1450.035.8243440
TATCAAC19200.035.7894022
TCAACGC20100.034.1868934
ATCAACG20350.033.7669073
CAACGCA20450.033.4337775
TCTCGTA1650.031.42364738
AACGCAG22100.030.9375926
ACGCAGA23200.029.4665477
CGCAGAG23200.029.4665478
ATTACGT605.168123E-428.98476845
AGAGTAC23800.028.72369211
GCCGTCT1850.028.22164246
CAGAGTA24250.028.19067410
CGTATGC1850.028.09860441
ATGCCGT1900.027.4356444
CTTAACG657.701022E-427.07366458
Produced by FastQC (version 0.11.5)