FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705771
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16630.23562883711572166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA350.00759981851.32422670
GGTATCA7500.042.645851
GTATCAA17200.038.5908161
GTTAGAC557.684832E-637.422613
CATGCGT400.002674741434.2894812
ATCAACG19700.033.7816663
TATCAAC19750.033.572422
TCAACGC19850.033.5168844
CAACGCA20250.032.8501665
AACGCAG21200.031.2163666
TAACGCT553.3719273E-431.1766724
TACCGTC450.00476194930.4795347
ACGCAGA21900.030.2185847
CGCAGAG21800.030.199918
AGAGTAC22400.028.93174711
CAGAGTA23100.028.50034510
GTGGTAT2700.028.0229591
GCAGAGT24250.027.2901829
AGTACGG18300.026.7945113
GTTCTAT658.8139065E-426.4552421
Produced by FastQC (version 0.11.5)