EGAF00005755699 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005755699
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7504
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	56

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT	208	2.771855010660981	No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	29	0.38646055437100213	RNA PCR Primer, Index 26 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGT	29	0.38646055437100213	No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT	28	0.3731343283582089	RNA PCR Primer, Index 26 (100% over 24bp)
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC	16	0.21321961620469082	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGG	14	0.18656716417910446	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.15991471215351813	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.15991471215351813	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.15991471215351813	No Hit
ACCTTGAAGCGCGACTGGTACTCGGCCAGAAAGTCGAGGGGATTGCCCTC	11	0.14658848614072495	No Hit
GTTCTCGACAGCTTGACCCAGCTGGACGGTGAGGGCGGTTTGCTCTGTCA	11	0.14658848614072495	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	10	0.13326226012793177	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.13326226012793177	No Hit
GTATAAAGTATCTTAGCTTTGCACCATAGAGGAAAAGTAGCGTGATAAGTATAACTCCGTAAGTTATGGTGGTTC	9	0.1199360341151386	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.1199360341151386	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.1199360341151386	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.1199360341151386	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.10660980810234541	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATGTCGTATGCCGT	8	0.10660980810234541	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTTTGCCGT	8	0.10660980810234541	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGGCGT	8	0.10660980810234541	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.10660980810234541	No Hit
GGTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG	8	0.10660980810234541	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.10660980810234541	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	30	8.6998625E-8	120.95959	70
AAGGGGG	30	2.1163396E-6	71.92192	69
GTATGCC	30	5.517366E-6	61.410255	42
TATGCCG	30	5.517366E-6	61.410255	43
CGTATGC	30	5.7790985E-6	60.94147	41
TCGTATG	30	6.3335283E-6	60.02506	40
CTTCTGC	25	1.5913158E-4	59.875	51
GTCTTCT	25	1.6547629E-4	59.41085	49
TCTCGTA	30	6.9313664E-6	59.135803	38
CGTCTTC	25	1.720206E-4	58.953846	48
TTCTGCT	20	0.0049392562	56.574806	52
TCTGCTT	20	0.0049392562	56.574806	53
CTGCTTG	20	0.0049392562	56.574806	54
AAAGGGG	40	1.9667214E-5	54.43182	70
TGCTTGA	35	1.19497E-5	53.880764	55
GCTTGAA	35	1.19497E-5	53.880764	56
TTGAAAA	35	1.19497E-5	53.880764	58
CTTGAAA	35	1.19497E-5	53.880764	57
TGCCGTC	35	1.3759367E-5	52.637363	45
ATGCCGT	35	1.3759367E-5	52.637363	44

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content