FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7504
Sequences flagged as poor quality0
Sequence length20-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6568.742004264392325No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG150.19989339019189767No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.14658848614072495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.14658848614072495No Hit
ACCTTGAAGCGCGACTGGTACTCGGCCAGAAAGTCGAGGGGATTGCCCTC100.13326226012793177No Hit
GTATCAACGCAGAGTACGGGGTGCCGATGCCATCGGGCTTGTCCGTCGTGGCGAGCTTGGGCTCGATGTAGCGCA90.1199360341151386No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90.1199360341151386No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATACC80.10660980810234541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80.10660980810234541No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGA200.0052098655.83778859
GGTATCA809.4989446E-832.511
TACGGGT500.00970561826.00800115
GTATCAA1308.390998E-925.0076921
TATCAAC1308.390998E-925.0076922
CAGAGTA1351.254557E-824.08148210
AGAGTAC1351.254557E-824.08148211
GCAGAGT1351.254557E-824.0814829
AACGCAG1401.8471837E-823.2214286
ACGCAGA1401.8471837E-823.2214287
AGTACGG1401.8471837E-823.22142813
GAGTACG1401.8471837E-823.22142812
ATCAACG1452.6822818E-822.420693
CGCAGAG1503.8438884E-821.6733348
GTACGGG1503.8438884E-821.67333414
TCAACGC1555.4418706E-820.9741944
CAACGCA1651.0545591E-719.703035
Produced by FastQC (version 0.11.5)