FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382823
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC16570.4328371074883171No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT16160.4221271971642247No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAGAAAGGGGGGTG15120.3949605953665271No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14770.3858179889923019No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13080.34167226107104326No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG12900.33697034922144176No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATC12640.33017869877201733No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC12360.32286461367263725No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12230.31946878844792503No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCGTTGACATT11180.29204096932524953No Hit
CTCCTGCACTGGAACCAGCGTTGACATTGGTGATCAAAGGCATGTCTCCTGGTATCAACAACACCCAGGCAACGT11120.29047366537538233No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC10870.2839432322509358No Hit
GGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10480.27375575657679924No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG10430.2724496699519099No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG10330.26983749670213125No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCGTTGACATTGGTGATCAAAGGCATGTCTCCTGGTATCAACAACA10290.2687926274022198No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAGA9820.2565154131282603No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9460.24711158942905728No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAGAAAGGGGGGTGA9340.24397698152932296No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT9200.24031993897963289No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG9180.23979750432967717No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC8780.22934881133056267No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG8100.21158603323206807No Hit
CCTATGAACATTCTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT8020.2094962946322452No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC7720.20165977488290934No Hit
GACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT7650.19983125360806428No Hit
GTCTGAGGACGAGGCTGATTATTATTGCAGCTCATTTATAACCAGAAGCATCACCCCCCTTTCTGTCTTCGGAA7260.1896437779339277No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC7130.1862479527092155No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGC6820.17815021563490177No Hit
TCTCTGGGCTCCAGTCTGAGGACGAGGCTGATTATTATTGCAGCTCATTTATAACCAGAAGCATCACCCCCCTTT6770.17684412901001245No Hit
CTCTCGGGGCGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC6660.17397073843525598No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG6650.17370952111027813No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT6470.1690076092606766No Hit
GGTATCAACAACACCCAGGCAACGTCCCCAAAGTCATCATTTATGATGTT6200.16195474148627434No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCT5720.14941630988733698No Hit
GCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCAC5710.14915509256235912No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA5620.14680413663755834No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA5580.1457592673376469No Hit
TCCTCAGACTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGACTAGAGACC5570.14549805001266902No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC5570.14549805001266902No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGACT5510.14393074606280187No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC5360.14001248618813394No Hit
GACCATCTCTGGGCTCCAGTCTGAGGACGAGGCTGATTATTATTGCAGCTCATTTATAACCAGAAGCATCACCCC5360.14001248618813394No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG5300.13844518223826677No Hit
GGTTATAAATGAGCTGCAATAATAATCAGCCTCGTCCTCAGACTGGAGCC5160.1347881396885767No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC5150.13452692236359884No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG5100.13322083573870952No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5080.1326984010887538No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC5060.1321759664387981No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACT5000.1306086624889309No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT4940.12904135853906373No Hit
GATTATTATTGCAGCTCATTTATAACCAGAAGCATCACCCCCCTTTCTGT4920.12851892388910802No Hit
CAACAACACCCAGGCAACGTCCCCAAAGTCATCATTTATGATGTTGATAATCGGCCCTCAGGGGTCTCTAGTCG4910.12825770656413016No Hit
ATAATAATCAGCCTCGTCCTCAGACTGGAGCCCAGAGATGGTCAGGGAGG4900.1279964892391523No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGCAGGGGCCA4880.12747405458919656No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG4800.1253843159893737No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG4790.12512309866439583No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGA4630.12094362146475002No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG4490.11728657891505996No Hit
GAGTTGGGAACCTATGAACATTCTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG4400.1149356229902592No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTGGTAGACGTCAACCTCAGGAAGAACTGATTCATCGACTTGTCAATTGA4390.11467440566528134No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG4330.11310710171541419No Hit
GTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCGTTGACATTGGTGATCAAAGGCATGT4290.11206223241550271No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC4270.111539797765547No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGG4250.11101736311559128No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA4250.11101736311559128No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT3990.10422571266616688No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC3990.10422571266616688No Hit
TCCCCAAAGTCATCATTTATGATGTTGATAATCGGCCCTCAGGGGTCTCT3980.10396449534118901No Hit
CTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCGCC3970.10370327801621115No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC3950.10318084336625541No Hit
TTTATAACCAGAAGCATCACCCCCCTTTCTGTCTTCGGAACTGGGACCAA3930.10265840871629969No Hit
GGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGT3920.10239719139132183No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGT3900.10187475674136612No Hit
AATCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCG3860.10082988744145467No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT3860.10082988744145467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)