FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881007
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22800.041.9197271
TCAACGC27250.035.074124
ATCAACG27650.034.566723
CAACGCA27750.034.5660485
ACGTATT400.002587437434.52325437
TACGTAT400.00259467834.5035936
GGTATCA9700.034.0257721
TATCAAC29150.033.37772
AACGCAG28850.033.3672756
ACGCAGA31150.030.7896947
CGCAGAG31250.030.6911668
AGAGTAC31400.030.32559611
CAGAGTA32050.029.81782210
AGTACGG27300.029.09094413
GCAGAGT32900.029.0474559
TTATCGT605.185487E-428.96916645
GAGTACG27500.028.87773512
GTGGTAT4750.026.7803731
GTACGGG30350.025.82763514
GTATTAA1608.185452E-1125.7851561
Produced by FastQC (version 0.11.5)